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AFP (Alpha Fetoprotein) ELISA kit


AFP (Alpha Fetoprotein) ELISA kit

Category Name Cancer ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle Sandwich Complex
Detection Range 0-300 ng/mL
Sample 20l serum
Specificity 98.5%
Sensitivity 2.0 ng/mL
Total Time ~80min
Shelf Life 12-14 Months from the manufacturing date

Item #:                    5101-16   Quantity:               

AFP (Alpha Fetoprotein) ELISA kit


AFP (Alpha Fetoprotein) ELISA kit

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AFP (Alpha Fetoprotein) ELISA kit description:

Materials Provided with AFP (Alpha Fetoprotein) ELISA Kit:

1. AFP ELISA Antibody-coated 96 wells Microplate with 96 wells.
2. Zero buffer, 12 ml
3. Reference standard set, contains 0, 5, 20, 50, 150 & 300 ng/ml
4. AFP EIA Kit Enzyme Conjugate Reagent, 18 ml
5. TMB Substrate, 12ml
6. AFP Elisa Test Stop Solution, 12 ml
7. Wash Buffer Concentrate(50X),15ml

Materials & Instrumentations required but not included with AFP ELISA kit:

- Precision pipettes 5~40 l,50~200l and 1.0 ml
- Distilled water
- Vortex mixer or equivalent
- Absorbent paper or paper towel
- Graph paper
- EIA kit Microplate Washer
- EIA kit Microplate Reader at 450nm

AFP ELISA Test Principle

Diagnostic Automation/Cortez Diagnostics, Inc. offers an AFP (Alpha Fetoprotein) ELISA kit which is an enzyme immunoassay for the quantitative measurement of AFP concentration in human serum. The AFP ELISA kit, is for diagnostic laboratory use only. The AFP ELISA kit is based on a solid phase enzyme-linked immunosorbent assay. The AFP ELISA kit utilizes one anti-AFP antibody for solid phase (microtiter wells) immobilization and another with mouse monoclonal anti-AFP antibody in the antibody-enzyme (horseradish peroxidase) conjugate solution. In the AFP ELISA kit, the test specimen (serum) is added to the AFP antibody coated microtiter wells and incubated with the Zero Buffer. If human AFP is present in the specimen, it will combine with the antibody on the well. The well is then washed to remove any residual test specimen, and AFP antibody labeled with horseradish peroxidase (conjugate) is added. The conjugate will bind immunologically to the AFP on the well, resulting in the AFP molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation at room temperature, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of 2N HCl, and the color is changed to yellow and measured spectrophotometrically at 450 nm. The concentration of AFP is directly proportional to the color intensity of the test sample. The sensitivity of the AFP ELISA kit is 2.0 ng/ml and the specificity is 97%.

AFP ELISA Test Background Information

Alpha-fetoprotein (AFP) is a glycoprotein with a molecular weight of approximately 70,000 daltons. AFP is normally produced during fetal and neonatal development by the liver, yolk sac, and in small concentrations by the gastrointestinal tract. After birth, serum AFP concentrations decrease rapidly, and by the second year of life and thereafter only trace amounts are normally detected in serum. Elevation of serum AFP to abnormally high values, occurs in several malignant diseases, most notably non-seminomatous testicular cancer and primary hepatocellular carcinoma. In the case of non-seminomatous testicular cancer, a direct relationship has been observed between the incidence of elevated AFP levels and the stage of disease. Elevated AFP levels have also been observed in patients diagnosed with seminoma with non-seminomatous elements, but not in patients with pure seminoma. In addition, elevated serum AFP concentrations have been measured in patients with other noncancerous diseases, including ataxia telangiectasia, hereditary tyrosinemia, neonatal hyperbilirubinemia, acute viral hepatitis, chronic active hepatitis, and cirrhosis. Elevated serum AFP concentrations are also observed in pregnant women. Therefore, the AFP ELISA test kit measurements are not recommended for use as a screening procedure to detect the presence of cancer in the general population.

Diagnostic Automation/Cortez Diagnostics, Inc. also provides other Cancer ELISA Kits. For more information about these ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030.