AFP (Alpha Fetoprotein) ELISA kit
|Category Name||Cancer ELISA kits|
|Method||ELISA method: Enzyme Linked Immunosorbent Assay|
|Detection Range||0-300 ng/mL|
|Shelf Life||12-14 Months from the manufacturing date|
Diagnostic Automation/Cortez Diagnostics, Inc. offers an AFP (Alpha Fetoprotein) ELISA kit which is an enzyme immunoassay for the quantitative measurement of AFP concentration in human serum. The AFP ELISA kit, is for diagnostic laboratory use only. The AFP ELISA kit is based on a solid phase enzyme-linked immunosorbent assay. The AFP ELISA kit utilizes one anti-AFP antibody for solid phase (microtiter wells) immobilization and another with mouse monoclonal anti-AFP antibody in the antibody-enzyme (horseradish peroxidase) conjugate solution. In the AFP ELISA kit, the test specimen (serum) is added to the AFP antibody coated microtiter wells and incubated with the Zero Buffer. If human AFP is present in the specimen, it will combine with the antibody on the well. The well is then washed to remove any residual test specimen, and AFP antibody labeled with horseradish peroxidase (conjugate) is added. The conjugate will bind immunologically to the AFP on the well, resulting in the AFP molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation at room temperature, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of 2N HCl, and the color is changed to yellow and measured spectrophotometrically at 450 nm. The concentration of AFP is directly proportional to the color intensity of the test sample. The sensitivity of the AFP ELISA kit is 2.0 ng/ml and the specificity is 97%.