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Aflatoxin B1 ELISA kit

Name

Aflatoxin B1 ELISA Test

Full name

Food Aflatoxin B1 ELISA Test Kit

Category Name Food ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA principle- Peroxidase conjugated
Detection Range Qualitative elisa assay- Positive, Negative Controls
Sensitivity 5pg /mL
Total Time 140 min.

Item #:                    5120-8   Quantity:               

Aflatoxin B1 ELISA kit

 

Aflatoxin B1 ELISA kit

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Aflatoxin B1 ELISA kit description:




Diagnostic Automation Inc. offers an Aflatoxin B1 Food ELISA Kit that is an Enzyme Immunoassay for the Quantitative determination of Aflatoxin B1 in Food. This Aflatoxin B1 Food ELISA Kit is a quick, economical, and sensitive Food Toxin ELISA Kit which is designed to detect Aflatoxin B1 in food.




Materials Provided with Aflatoxin B1 Elisa Kit:
1. Microtiter plate: 12 strips with 8 breakable wells coated with aflatoxin conjugate
2. Aflatoxin B1 Standards (0; 100; 400; 1000; 4000; 10000 pg/mL): 6 vials
3. Anti-Aflatoxin B1 Antibody (rabbit): 6 mL
4. Conjugate (anti-rabbit-IgG-HRP): 15 mL
5. Substrate Solution (TMB): 15 mL
6. Stop Solution (0.5 M H2SO4): 15 mL
7. Sample/Standard Diluent (PBS): 60 mL.
8. Washing Solution (PBS + Tween 20): 60 mL as 10x
9. Two plastic foils to cover the strips during the incubation
10. Plastic bag to store unused microtiter strips
11. Instruction Manual

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength



Aflatoxin B1 Food ELISA Kit Background Information:
Aflatoxins belong to the class of mycotoxins. Chemically they are defined as difuranocyclopentanocumarines or difuranopentanolidocumarines, i.e. aflatoxins contain a dihydrofuran or a tetrahydrofuran ring, to which a substituted cumarin system is condensed. Out of about 20 known aflatoxins, the moulds Aspergillus flavus and A. parasiticus produce exclusively aflatoxin B1, B2, G1 and G2, and all the other aflatoxins are derivates of these four. The derivates are developed either by metabolism in humans, animals and microorganisms or by environmental reactions. Aflatoxins are the strongest mycotoxins, which act primarily in a hepatotoxic and carcinogenic way. The four main aflatoxins show a different toxicity. B1 is without doubt the most toxic aflatoxin, followed by G1, B2 and G2. Both chronical and acute intoxications are effected by aflatoxins.



Aflatoxin B1 Food ELISA Test Principle:
The Aflatoxin B1 Food ELISA Kit is a quantitative test based on the principle of the enzyme linked immunosorbent assay. An aflatoxin conjugate is bound on the surface of a microtiter plate. Immobilized and free aflatoxin B1 compete for the antibody binding sites.




Diagnostic Automation Inc. offers several popular Food ELISA Test Kits: Chloramphenicol ELISA Kit, Fumonisin ELISA Kit, Streptomycin ELISA Kit, Aflatoxin M1 ELISA Kit, Penicillin ELISA Kit, Histamine ELISA Kit, and Verotoxin Antigen in Food ELISA Test Kit. For more information on any of these Food ELISA Test Kits please see our website home page, or contact our Customer Service Representative at 818-591-3030.


Product Note:

Diagnostic Automation, Inc. Aflatoxin B1 ELISA is a quick, economical and sensitive method to detect aflatoxin B1 in food. After an appropriate sample preparation, 40 samples can be tested in duplicate within 140 minutes.Some additional instrumentation and reagents are needed when running DAI Aflatoxin B1 quantitative test kit which include,  50, 100, 500 and 1000 uL-micropipets, Microtiter plate shaker, ELISA reader (450 nm), Mortar or mixer,  Horizontal shaker or magnetic stirrer, Centrifuge, Methanol and Double-distilled water.