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Human TPO ELISA Test Kit

Category Name Autoimmune Disease kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA - Indirect; Antigen Coated Plate
Detection Range Qualitative/Semi-Quantitative elisa assay: Positive, negative, cut off
Sample 10ul
Specificity 94.4%
Sensitivity 93.70%
Total Time ~60 min
Shelf Life 12 Months from the manufacturing date

Item #:                    1446-P1   Quantity:               


TPO IgG 1446-2 (MSDS) 2017-2-7.pdf


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TPO ELISA kit description:

The Diagnostic Automation Inc. TPO IgG ELISA test kit is a qualitative and semi-quantitative detection of IgG-class antibody to thyroid peroxidase (TPO) in human serum. The TPO test kit system is intended to be used as an aid in the diagnosis of thyroid diseases.

Materials provided with the TPO IgG ELISAtest kit:
1. Plate: strips coated with purified human thyroid peroxidase
2. HRP-Conjugate: Conjugated HRP-goat anti-human IgG (Fc chain specific)
3. Positive Control: Human Serum
4. Calibrator A: Human Serum
5. Calibrator B: Human Serum
6. Calibrator C: Human Serum
7. Calibrator D: Human Serum
8. Negative Control: Human Serum
9. Sample Diluent
10. TMB
11. Stop Solution
12. Wash Buffer Concentrate (10X)

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength

TPO IgG ELISATest Background Information:
Thyroid antibodies are a characteristic finding in patients with Hashimoto and Graves diseases. The presence of thyroid antibodies in the sera of 80% of patients with these two diseases led to the recommendation that some type of thyroid antibody testing be a feature of the work-up of any patient with a goiter. Although thyroid antibodies are predominantly associated with Hashimoto or Graves diseases, they may be found in the sera of patients with other diseases such as myxedma, granulomatous thyroiditis, nontoxic nodular goiter, and thyroid carcinoma. Thyroid antibodies are also found in most cases of lymphocytic thyroiditis in children, and rarely in patients with pernicious anemia and Sjogren s Syndrome.

TPO IgG ELISA Test Principle:
(TPO) ELISA test system is designed to detect IgG class antibodies to TPO in human sera. Wells of plastic microwell strips are sensitized by passive absorption with TPO antigen. The thyroid peroxidase test procedure involves three incubation steps.

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Notable features of Autoimmune Disease ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Autoimmune Disease ELISA kits follow a similar method. See the TPO IgG ELISA kit product insert for details on preparation, procedures, quality control, and test result interpretation.

For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.