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Intact PTH ELISA kit

Name

Intact PTH ELISA Test

Full name

Human Intact PTH ELISA Test Kit

Category Name Bone Metabolism ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle Sandwich Complex
Detection Range 1.57 - 210 pg/mL
Sample 25 uL Serum
Specificity 97%
Sensitivity 1.57
Total Time ~ 3 hours 30 min
Shelf Life 12 months from the manufacturing date

Item #:                    1735-6   Quantity:               

Intact PTH ELISA kit

Intact _ELISA_1735-6(MSDS)(11-21-2014).pdf

Intact PTH ELISA kit

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Intact PTH ELISA kit description:




Diagnostic Automation Intact-PTH ELISA is intended for the quantitative determination of Intact-PTH (Parathyroid Hormone) in human serum.



Material Provided with Intact PTH ELISA Kit:
1. RGT 1: Biotinylated PTH Antibody
2. RGT 2: Peroxidase Enzyme labeled PTH Antibody
3. RGT B: TMB Substrate
4. RGT 3: Diluent (equine serum)
5. RGT A: ELISA Wash (Saline with surfactant)
6. SOLN: Stopping Solution (1 N sulfuric acid)
7. RGT 4: Reconstitution Solution containing surfactant
8. PLA: Microplates, wells coated with Streptavidin
9. CAL: Lyophilized synthetic h-PTH A: 0 pg/mL B-F: Refer to vial
10. CTRL: Controls 1 & 2

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm




Intact PTH ELISA Kit Background Information:
Intact PTH assays are important for the differentiation of primary hyperparathyroidism from non-parathyroid-mediated forms of hypercalcemia, such as malignancy, sarcoidosis and thyrotoxicosis. The measurement of parathyroid hormone is the most specific way of making the diagnosis of primary hyperparathyroidism. In the presence of hypercalcemia, an elevated level of parathyroid hormone virtually establishes the diagnosis. In over 90% of patients with primary hyperparathyroidism, the parathyroid hormone will be elevated. Intact PTH is biologically active and clears very rapidly from the circulation with a half-life of less than four minutes. PTH undergoes proteolysis in the parathyroid glands, but mostly peripherally, particularly in the liver but also in the kidneys and bone, to give N-terminal fragments and longer lived C-terminal and mid-region fragments. In subjects with renal insufficiency, C-terminal and mid-region PTH assays typically give elevated PTH results, as reflected by impaired renal clearance.




Intact PTH ELISA Test Principle:
The Diagnostic Automation Intact PTH Immunoassay is a two-site ELISA assay for the measurement of the biologically intact 84 amino acid chain of PTH. Two different goat polyclonal antibodies to human PTH have been purified by affinity chromatography to be specific for well-defined regions on the PTH molecule. One antibody is prepared to bind only the mid-region and C-terminal PTH 39-84 and this antibody is biotinylated. The other antibody is prepared to bind only the N-terminal PTH 1-34 and this antibody is labeled with HRP for detection. For additional details please refer to the instructions for use.




Notable features of the Bone Metabolism ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for all Bone Metabolism ELISA kits follow a similar method. See the Intact PTH ELISA Kit product insert for specific details on preparation, procedures, quality control, and test result interpretation.

Diagnostic Automation Inc. also provides other Bone Metabolism ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .




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