Chloramphenicol ELISA kit description:

Diagnostic Automation/Cortez Diagnostics, Inc. offers a Chloramphenicol Food ELISA Kit that is an Enzyme Immunoassay for the Quantitative Determination of Chloramphenicol in Food.  This Chloramphenicol Food ELISA Kit is a quick, economical, and sensitive Food Toxin ELISA Kit which is designed to detect Chloramphenicol in food.  The Chloramphenicol Food ELISA Kit represents a highly sensitive Food ELISA Test Kit detection system and is particularly capable of the quantification of chloramphenicol in food products.

 

The Chloramphenicol Food ELISA Kit is a quantitative test based on the principle of the enzyme-linked immunosorbent assay.  An antibody binding protein is coated on the surface of a microtiter plate on the Chloramphenicol ELISA Kit.  Chloramphenicol containing samples or standards and an antibody directed against chloramphenicol are given into the wells of the microtiter plate on the Chloramphenicol ELISA kit.  The chloramphenicol contained in samples or standards will bind to the antibody which reacts with the binding protein coated onto the microtiter plate of the Chloramphenicol ELISA kit.  After 30 minutes incubation at room temperature, a chloramphenicol-peroxidase conjugate is added into the wells of the Chloramphenicol ELISA kit without a preceding washing step to saturate free antibody binding sites. After additional 15 minutes incubation at room temperature, the wells of the Chloramphenicol ELISA test are washed with diluted washing solution to remove unbound material. A substrate solution is added to the Chloramphenicol ELISA test and incubated for 15 minutes, resulting in the development of a blue color on the Chloramphenicol ELISA test kit. The color development on the Chloramphenicol ELISA test is inhibited by the addition of a stop solution, and the color turns yellow. The yellow color is measured photometrically at 450 nm. The concentration of chloramphenicol on this Chloramphenicol ELISA test kit is indirectly proportional to the color intensity of the Chloramphenicol Food ELISA test sample.

Due to its outstanding antibacterial properties, chloramphenicol is an often used antibiotic in the production of milk, meat and eggs. In humans it leads to haematotoxic side effects like the chloramphenicol induced aplastic anemia. This has caused low limits in Germany, e.g. 1 μg/kg for milk, meat and eggs.  Untill now, the chloramphenicol concentration was determined by radioimmunoassay or by gas chromatography. However, compared with conventional methods, enzyme immunoassays show some essential advantages. There is no need to work with radioactive material; the required assay time on this Chloramphenicol Food ELISA Test Kit is shorter and the sensitivity is better than with chromatographic methods.  Forty samples on this Food ELISA test kit can be assayed in duplicate within 60 minutes.

Diagnostic Automation/Cortez Diagnostics, Inc. offers several popular Food ELISA Test Kits:   Fumonisin ELISA Kit, Streptomycin ELISA Kit, Aflatoxin B1 ELISA Kit, Aflatoxin M1 ELISA Kit, Penicillin ELISA Kit, Histamine ELISA Kit, and Verotoxin Antigen in Food ELISA Test Kit.  For more information on any of these Food ELISA Test Kits, please contact our Customer Service Department.