Cortisol Saliva Elisa kit description:

Cortisol Saliva ELISA kit is for the quantitative determination of Cortisol by enzyme immunoassay in human saliva.




Material Provided with Cortisol Saliva ELISA kit:
1. Test Strips Microwells: 96 test wells with Rabbit Anti-Cortisol Antibody
2. Cortisol-HRP Conjugate
3. Cortisol Saliva Calibrators
4. Controls
5. Wash Buffer
6. Assay Buffer
7. TMB Substrate
8. Stopping Solution

Materials Required, Not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm filter




Cortisol ELISA kit Background Information:
Cortisol is the most abundant circulating steroid and the major glucocorticoid secreted by the adrenal cortex. Cortisol is physiologically effective in blood pressure maintenance and anti-inflammatory activity. It is also involved in calcium absorption, gluconeogenesis as well as the secretion of gastric acid and pepsin. It is increased under stress situations, physical exercise and
external administration of ACTH. Measurement of cortisol levels in general can be used as an indicator of adrenal function and the differential diagnosis of Addison and Cushing diseases as well as adrenal hyperplasia and carcinoma. Most circulating cortisol is bound to cortisol binding globulin or transcortin and albumin. The free cortisol, which is considered the active part of blood, is about 1-2%. In the absence of appreciable amounts of the cortisol binding proteins in saliva , salivary cortisol is considered to be free and shows a diurnal rhythm with the highest levels in the morning and the lowest levels at night.




Cortisol ELISA Test Principle:
The principle of the Cortisol Saliva ELISA test follows the typical competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. For additional details please refer to the instructions for use.


Product inserts for most Steroid ELISA kits follow a similar method. See the Cortisol saliva ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.



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