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Dengue IgG ELISA Test

Name

Dengue IgG ELISA kit

Full name

Human Dengue IgG ELISA Test Kit

Category Name Parasitology ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA principle - Indirect; Antigen Coated Plate
Detection Range Semi-quantitative elisa assay - Positive, Negative Controls
Sample 10 ul
Specificity 97.4%
Sensitivity 94.7%
Total Time ~25min
Shelf Life 12 Months from the manufacturing date

Item #:                    8116-35   Quantity:               

Dengue IgG ELISA Test

 

Dengue IgG ELISA Test

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Dengue IgG ELISA Test description:




The Diagnostic Automation, Inc. Dengue IgG ELISA test is a semi-quantitative enzyme immunoassay for the detection of antibodies to dengue, in samples of human serum or plasma.

Materials provided with Dengue IgG Elisa test kit :

1. Plate: Microwells containing dengue antigens (serotype 1-4) - 96 test wells in a test strip holder
2. Enzyme Conjugate: One bottle containing 11 ml of anti-human IgG (gamma chain specific) conjugated to peroxidase
3. Positive Control: One vial containing 1 ml of diluted positive human serum
4. Negative Control: One vial containing 1 ml of diluted negative human serum
5. Chromogen: One bottle containing 11 ml of chromogen TMB
6. Wash Concentrate 20X: One bottle containing 25 ml of concentrated buffer and surfactant.
7. Dilution Buffer: Two bottles containing 30 ml of buffered protein solution
8. Stop Solution: One bottle containing 11 ml of 1 M phosphoric acid


Materials required but not provided:

1. Micropipette
2. Squeeze bottle for washing strips
3. Reagent grade (DI) water
4. Graduated Cylinder
5. Sample Dilution Tubes
6. Absorbent paper
7. ELISA plate reader
8. ELISA plate reader with a 450 nm & a 620-650 nm filter


Dengue IgG Elisa Kit Background Information
Dengue fever, caused by any of the four serotypes of dengue virus, is endemic in Southeast Asia as well as South and Central America. Repeat infection with a second type of dengue virus is thought to cause dengue hemorrhagic fever in about 10 percent of infected people. Dengue antibodies do not confer immunity beyond 3-6 weeks to a second dengue type. Symptoms of classical dengue fever, following a 5-8 day incubation period, include rash, severe headache, nausea, vomiting, chills, malaise, macular rash and may include lymphadenopathy. Hemorrhagic dengue fever involves increased blood vessel permeability which can lead to shock and death in about 10% of reported cases. Dengue fever can only be treated by supportive care and is prevented by mosquito control. In primary infections, circulating IgM antibody to the viral coat proteins is detected 5-6 days after the onset of illness, and gradually decreases within 1-2 months of onset. IgG antibody to dengue virus is detected approximately 14 days after onset in primary infections, and by day 2 in secondary infections. In secondary infections, IgM antibody may reappear but gradually diminishes, while IgG antibody persists, often at high titer. These patterns of dengue antibody development permit serological differentiation of primary and secondary infections. Characteristically, acute patients with primary infections have a higher IgM:IgG ratio than are found in secondary infections. Patients with secondary infections generally have higher IgG levels. Acute or recent infections are identified by a rise in antibody titer as well as high IgM levels.


Dengue IgG Elisa Test Principle
The microwells are coated with purified dengue virus antigen from cell cultured type 1-4 dengue. During the first incubation with the diluted patients’ sera, any antibodies which are reactive with the antigen will bind to the coated wells. After washing to remove the rest of the sample, the Enzyme Conjugate is added. If antibodies have been bound to the wells, the Enzyme Conjugate will then bind to these antibodies. After another series of washes, a chromogen (tetramethylbenzidine or TMB) is added. If the Enzyme Conjugate is present, the peroxidase will catalyze a reaction that consumes the peroxide and turns the chromogen from clear to blue. Addition of the Stop Solution ends the reaction and turns the blue color to a bright yellow color. The reaction may then be read visually or with an ELISA reader.


Dengue IgG Elisa Kit Performance Characteristics
Positive Agreement: 94.7% (18/19)
Negative Agreement: 97.4% (75/77)


Dengue IgG Elisa Test Results Interpretation
Initially Non-reactive: Samples interpreted as non-reactive (0.0-0.15 OD units, or zero color) indicate antibody is not present in the sample. Since antibody may not be present during early disease, (5-8 days incubation), confirmation 2-3 weeks later is indicated for laboratory diagnosis.
Initially Reactive: Samples interpreted as strongly reactive (>1.0 OD or +++) may indicate the presence of specific antibody. For further details please refer to complete instructions manual supplied with the product. Product inserts for most Parasitology ELISA kits follow a similar method.


Dengue IgG Related ELISA Kits:
Dengue ELISA Kits are available in several formats: Dengue IgG ELISA Kit, Dengue IgG/IgM ELISA Kit, and Dengue NS1 ELISA Kit for detection of NS1 antigens in human serum.

1. Dengue IgM ELISA Kit
http://www.rapidtest.com/index.php?i=Dengue-IgM-ELISA-kit&id=172&cat=17

2. Dengue IgG/IgM ELISA Kit
http://www.rapidtest.com/index.php?i=Dengue-IgG/IgM-ELISA-kit&id=171&cat=17

3. Dengue NS1 ELISA Kit
http://www.rapidtest.com/index.php?i=Dengue-NS1-ELISA-kit---Antigen&id=682&cat=17

Diagnostic Automation Inc. also provides other Parasitology ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.

Product Note:

Diagnostic Automation, Inc. Microwell ELISA Dengue Fever IgG test kit  should be performed on serum.

Serological specimens should be collected under aseptic conditions. Hemolysis is avoided through prompt separation of the serum from the clot. Serum may be stored at 2-8 ºC for up to five days. Serum may be frozen below -20 ºC for 3-6 months.

Lipemic and strongly hemolytic serum should be avoided. Freezing whole blood

samples is not advised. Do not heat inactivate samples and avoid repeated freezing and thawing of samples.




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