our products
our products
information_top

Call us at:

(818)591-3030

USA

Click here to send us an email
tel_section

Diagnostic Automation Question & AnswerDiagnostic Automation Question & Answer Diagnostic Automation Articles Diagnostic Automation kit images Diagnostic Automation videos


Pregnancy Rapid tests

Leptin ELISA kit

Name

Leptin ELISA Test

Full name

Human Leptin ELISA Test Kit

Category Name Diabetes Assays ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA principle: Solid phase enzyme linked immunosorbent assay
Detection Range 0-600 U/L
Sample 10ul
Specificity Not Observed
Sensitivity 4.6U/ml
Total Time ~90min
Shelf Life 12months

Item #:                    1742-6   Quantity:               

Leptin ELISA kit

 

Leptin ELISA kit

callback phone number email us


Leptin ELISA kit description:




The DAI Leptin Enzyme Immunoassay Kit is used for the quantitative determination of Leptin in human serum and plasma.



Materials Provided with Leptin Elisa Test Kit:
Microtiter plate: 12x8 (breakable) strips, 96 wells coated with anti-Leptin monoclonal antibody
2. Standard (Standard 0-5): 6 vials ready to use
Concentrations: 0, 2, 5, 25, 50 and 100 ng/ml
3. Control: 2 vials ready to use; 2 levels (low and high)
4. Assay Buffer: 1 vial ready to use contains 0.3% Proclin as a preservative
5. Antiserum: 1 vial ready to use polyclonal Leptin antiserum
6. Enzyme Complex:1 vial ready to use Anti-rabbit complex conjugated to horseradish Peroxidase
7. Substrate Solution: 1 vial ready to use TMB
8. Stop Solution: 1 vial ready to use 0.5M H2SO4
9. Wash Solution: 1 vial 40X concentrated

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength
5. Quality Control Materials



Leptin Elisa Test Background Information:
Leptin is from the Greek word Leptos which means thin. This hormone is made by fat cells and regulates the amount of fat stored in the body. Leptin exerts this effect by adjusting both the sensation of hunger and of energy expeditures. Hunger is inhibited when the amount of fat stored reaches a certain level. Leptin is then secreted and circulates through the body, eventually activating leptin receptors in the hypothalamus. Energy expenditure is increased both by the signal to the brain, and directly via leptin receptors on peripheral targets. Although leptin reduces appetite as a circulating signal, obese individuals generally exhibit a higher circulating concentration of leptin than normal weight individuals due to their higher percentage body fats. These people show resistance to leptin, similar to resistance to insulin in type 2 diabetes. Leptin, also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells which have leptin receptors. Many of these additional functions are yet to be defined.



Leptin Elisa Test Principle:
The Diagnostic Automation Leptin ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal antibody directed towards a unique antigenic site on a Leptin molecule. An aliquot of patient sample containing endogenous Leptin is incubated in the coated well with a specific rabbit anti Leptin antibody. A sandwich complex is formed.After incubation the unbound material is washed off and an anti rabbit peroxidase conjugate is added for detection of the bound Leptin. Having added the substrate solution, the intensity of color developed is proportional to the concentration of Leptin in the patient sample.



For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.




Product Note:

Leptin is produced primarily in the adipocytes. It is also produced by brown adipocytes, placenta, ovaries, skeletal muscle, stomach, bone marrow, liver, pituitary gland. Leptin Elisa test kit is used in quantitative determination of Leptin both in serum and plasma.