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ENA Profile-6 ELISA kit

Name

ENA Profile ELISA Test

Full name

Human ENA Profile-6 ELISA Test Kit

Category Name Autoimmune Disease kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA principle- Peroxidase conjugated
Detection Range Sandwich Complex
Sample 10ul serum
Specificity 100%
Sensitivity 96%
Total Time ~65 min
Shelf Life 12 Months from the manufacturing date

Item #:                    2506-2   Quantity:               

ENA Profile ELISA_2506-2 (MSDS) (2017-2-7).pdf

ENA Profile-6 ELISA kit

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ENA Profile-6 ELISA kit description:




The Diagnostic Automation ENA Profile-6 ELISA test system is a semiquantitative
immunoassay for the detection of IgG antibodies to Jo-1, Sm, Sm/RNP, SSA(Ro), SSB(La), and Scl-70 in human sera. When performed according to these instructions, the results of this autoantibody profile may aid in the diagnosis and treatment of autoimmune connective tissue disorders.




Materials provided with the ENA Profile-6 ELISA test kit:
1. Plate: strips coated with inactivated antigen
Each row of the plate is coated with a different ENA
2. HRP-Conjugate: Conjugated HRP-goat anti-human IgG (Fc chain specific)
3. Positive Control: Human Serum
4. Calibrator: Human Serum
5. Negative Control: Human Serum
6. Sample Diluent
7. TMB
8. Stop Solution
9. Wash Buffer Concentrate (10X)

Materials required, not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength




ENA Profile-6 ELISA Test Background Information:
In recent years, it has become clear that autoantibodies to a number of nuclear constituents have proven to be useful in the diagnosis of various connective tissue diseases.The presence of high level RNP antibodies alone are considered diagnostic of mixed connective tissue disease (MCTD) and are usually associated with a more benign disease course, while patients with low levels of RNP antibodies, together with other autoantibodies, may be observed in the serum of patients with progressive systemic sclerosis, Sjogren s Syndrome, and rheumatoid arthritis. The presence of RNP antibodies in the serum of SLE patients is usually associated with a lower incidence of renal involvement and a more benign disease course. To the contrary, patients with Sm antibodies experience a higher frequency of renal and central nervous system complications.




ENA Profile-6 ELISA Test Principle:
The ENA Profile-6 ELISA test system is designed to detect IgG class antibody to different autoantigens in human sera. Wells of plastic microwell strips are sensitized by passive absorption with immobilized antigens. The test procedure involves three incubation steps.




Notable features of Autoimmune Disease ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results
Product inserts for most Autoimmune Disease ELISA kits follow a similar method. See the ENA Profile-6 ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.


For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.

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