HCG Visual ELISA kit
|Category Name||Fertility ELISA kits|
|Method||ELISA mehod: Enzyme Linked Immunosorbent Assay|
|Principle||ELISA principle- Peroxidase Conjugated Sandwich ELISA|
|Total Time||~ 20 min|
|Shelf Life||12-14 months|
The HCG Visual Test Kit is based on a solid phase enzyme-linked immunosorbent assay. The assay system utilizes one anti-HCG antibody for solid phase (microtiter wells) immobilization and another mouse monoclonal anti-HCG antibody in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test specimen (serum or urine) is added to the HCG antibody coated microtiterwells and incubated with the HCG antibody labeled with horseradish peroxidase (conjugate). If HCG is present in the specimen, the HCG molecules will be sandwiched between the solid phase and enzyme-linked antibodies. After incubation at room temperature, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB is added and incubated for five minutes, resulting in the development of a blue color. The color development is stopped with the addition of 2N HCl, and the color is changed to yellow and measured spectrophotometrically at 450 nm.