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Human LH ELISA Test Kit

Category Name Fertility ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle Sandwich Complex
Detection Range 0-200 mIU/mL
Sample 50uL Serum
Specificity 96%
Sensitivity 2.0 mIU/mL
Total Time ~80 min
Shelf Life 12 Months from the manufacturing date

Item #:                    4225-16   Quantity:               




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LH ELISA kit description:

The Diagnostic Automation LH EIA test kit is for the quantitative determination of human luteinizing hormone (LH) concentration in human serum.

Material Provided with LH ELISA Kit:
1. Antibody coated microtiter plate
2. LH EIA kit Reference standard set
3. Enzyme Conjugate Reagent
4. LH Elisa test TMB Substrate
5. Stop Solution
6. LH Elisa Kit Wash Buffer Concentrate 50X

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm

LH ELISA Kit Background Information:
Luteinizing hormone (LH) is produced in both men and women from the anterior pituitary gland in response to luteinizing hormone-releasing hormone (LH-RH or Gn-RH), which is released by the hypothalamus. LH, also called interstitial cell-stimulating hormone (ICSH) in men, is glycoprotein. In the differential diagnosis of hypothalamic, pituitary, or gonadal dysfunction, assays of LH concentration are routinely performed in conjugation with FSH assays since their roles are closely interrelated. Furthermore, the hormone levels are used to determine menopause, pinpoint ovulation, and monitor endocrine therapy.

LH ELISA Test Principle:
The LH Quantitative test kit is based on the principle of a solid phase enzyme linked immunosorbent assay. The assay system utilizes one anti-LH antibodyantibody for microtiter wells immobilization and a mouse monoclonal anti-HGH antibody in the antibody-enzyme horseradish peroxidase conjugate solution. The test sample is allowed to react simultaneously with the antibodies, resulting in LH molecules being sandwiched between the solid phase and enzyme-linked antibodies. For additional details please refer to the instructions for use.

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Diagnostic Automation Inc. also provides other Autoimmune Disease ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .