Diagnostic Automation/Cortez Diagnostics, Inc. offers a Penicillin Food ELISA Kit that is an Enzyme Immunoassay for the Quantitative Determination of Penicillin in Food. This Penicillin Food ELISA Kit is a quick, economical, and sensitive Food Toxin ELISA Kit which is designed to detect penicillin in food. The Penicillin Food ELISA Kit represents a highly sensitive Food ELISA Test Kit detection system and is particularly capable of the quantification of Penicillin in food products.
The Penicillin Food ELISA Kit is a quantitative test based on the principle of the enzyme linked immunosorbent assay. A penicillin conjugate is bound on the surface of a microtiter plate on the Penicillin ELISA Kit. Penicillin containing samples or standards and an antibody directed against penicillin are given into the wells of the microtiter plate on the Penicillin ELISA Kit. Immobilized and free penicillin compete for the antibody binding sites. After one hour incubation at room temperature, the wells of the Penicillin ELISA Kit are washed with diluted washing solution to remove unbound material. A peroxidase conjugate directed against the penicillin antibody is given into the wells of the Penicillin ELISA test and after another hour incubation, the plate is washed again. Then a substrate solution is added to the Penicillin ELISA test and incubated for 20 minutes, resulting in the development of a blue color. The color development on the Penicillin ELISA test is inhibited by the addition of a stop solution, and the color turns yellow. The yellow color is measured photometrically at 450 nm. The concentration of penicillin on this Penicillin ELISA test kit is indirectly proportional to the color intensity of the Penicillin Food ELISA test sample.
Penicillin was accidentally detected by Alexander Fleming in 1929. The drug belongs to the mycotoxins and is generated by the mould Penicillium chrysogenum. Penicillin as an antibiotic is preferentially used in the treatment of gram-positive bacteria, both for humans and animals. Of all illegally administered drugs, antibiotics are most frequently used in productive livestock. Contaminations in food or milk are ingested by humans, and can lead to severe infections by pathogen germs which became resistant against penicillin, or to allergies. The allergic reactions appear with different severity, dependent on dose and individual disposition, and showing symptoms from urticaria to anaphylactic shock. During routine testing of milk samples for antibiotics, in more than 90% of the positive cases, betalactam preparations or penicillins are detected. The method of choice for the determination of penicillin contamination in food has always been a microbiological assay. These procedures allow however no quantitative determination and no identification of the antibiotic drug, which is achieved by a sensitive Penicillin Food ELISA test kit or immunoaffinity columns together with HPLC.
Diagnostic Automation/Cortez Diagnostics, Inc. offers several popular Food ELISA Test Kits: Chloramphenicol ELISA Kit, Fumonisin ELISA Kit, Aflatoxin B1 ELISA Kit, Aflatoxin M1 ELISA Kit, Streptomycin ELISA Kit, ELISA Kit, Histamine ELISA Kit, and Verotoxin Antigen in Food ELISA Test Kit. For more information on any of these Food ELISA Test Kits, please contact our Customer Service Department.