Aflatoxin M1 ELISA kit (in Milk and Milk Products) description:

Diagnostic Automation/Cortez Diagnostics, Inc. offers an Aflatoxin M1 Food ELISA Kit, that is an Enzyme Immunoassay for the Quantitative Determination of Aflatoxin M1 in Milk and Milk Products.  This Aflatoxin M1 Food ELISA Kit is a quick, economical, and sensitive Food Toxin ELISA Kit which is designed to detect aflatoxin M1 in milk and milk products.  The Aflatoxin M1 Food ELISA Test Kit represents a highly sensitive Food ELISA Test Kit detection system and is particularly capable of the quantification of Aflatoxin M1 in milk and milk products.

 

The Aflatoxin M1 Food ELISA Kit is a quantitative test based on the principle of the enzyme linked immunosorbent assay. An aflatoxin conjugate is bound on the surface of a microtiter plate on the Aflatoxin M1 ELISA Kit.  Aflatoxin M1 containing samples or standards and an antibody directed against aflatoxin M1 are given into the wells of the microtiter plate on the Aflatoxin M! ELISA Kit.  Immobilized and free aflatoxin M1 compete for the antibody binding sites. After one hour incubation at room temperature, the wells of the Aflatoxin M1 ELISA Kit are washed with diluted washing solution to remove unbound material.  A peroxidase conjugate against the antibody is given into the wells of the Aflatoxin M1 ELISA kit and after another hour incubation, the plate is washed again. Then a substrate solution is added and incubated for 20 minutes, resulting in the development of a blue color on the Aflatoxin M1 ELISA test. The color development on the Aflatoxin M1 ELISA test is inhibited by the addition of a stop solution, and the color turns yellow. The yellow color is measured photometrically at 450 nm. The concentration of aflatoxin M1 on this Aflatoxin M1 ELISA test kit is indirectly proportional to the color intensity of the Aflatoxin M1 ELISA test sample.

Aflatoxins belong to the class of mycotoxins. Chemically they are defined as difuranocyclopentanocu- marines or difuranopentanolidocumarines, i.e. aflatoxins contain a dihydrofuran or a tetrahydrofuran ring, to which a substituted cumarin system is condensed.  Out of about 20 known aflatoxins, the moulds Aspergillus flavus and A. parasiticus produce exclusively aflatoxin B1, B2, G1 and G2, and all the other aflatoxins are derivates of these four. The derivates are developed either by metabolism in humans, animals and microorganisms or by environmental reactions.  Aflatoxin M1 was the first metabolite of Aflatoxin B1, which could unequivocally be detected by Allcroft and Carnaghan in the milk of cows in 1963.  Out of this reason this first derivative was called  Aflatoxin M1 (= milk). As further investigations showed, also other mammalians excrete Aflatoxin M1 in milk, feces and urine. Contaminations of milk and milk products can be hazardous for human beings, because M1 is similar to Aflatoxin B1 regarding its hepatotoxicity. M1 is only less carcinogenic. In order to protect people against aflatoxin-induced diseases, there is a need for the qualitative and quantitative control of endangered foodstuff, besides appropriate hygienic precautions, which avoid the formation of aflatoxins. After an appropriate sample preparation on this Aflatoxin M1 Food ELISA test kit, 40 samples can be tested in duplicate within 140 minutes.

Diagnostic Automation/Cortez Diagnostics, Inc. offers several popular Food ELISA Test Kits:  Fumonisin ELISA KIt, Chloramphenicol ELISA Kit, Streptomycin ELISA Kit, Aflatoxin B1 ELISA Kit, Penicillin ELISA Kit, Histamine ELISA Kit, and Verotoxin Antigen in Food ELISA Test Kit.  For more information on any of these Food ELISA Test Kits, please contact our Customer Service Department.