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Gluten ELISA Kit


Gluten ELISA Test

Full name

Food Gluten / Gliadin ELISA Test Kit

Category Name Food ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA principle- Peroxidase conjugated
Detection Range Qualitative elisa assay- Positive, Negative Controls
Sensitivity 0.3 ppm
Total Time 60 min.

Item #:                    5139-8   Quantity:               

Gluten ELISA Kit

Gluten ELISA Kit

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Gluten ELISA Kit description:

The DAI Gluten/ Gliadin ELISA test kit is an Enzyme Immunoassay for the Quantitative determination of Gluten in Food. Gluten/ Gliadin ELISA kit represents a highly sensitive detection system and is particularly capable of the quantification of gliadin/gluten residues in bakery products, baby food, meat, and chocolate.

Materials Provided with Gluten ELISA Test Kit:
1. Microtiter plate:12 strips with 8 breakable wells coated with anti-gliadin
2. Gliadin Standards (0, 2, 6, 20, 60 ppm Gliadin): 1 x 5 vials with 1.0 mL each
3. Conjugate (anti-gliadin-Peroxidase): 15 mL
4. Substrate Solution (TMB): 15 mL
5. Stop Solution (0.5 M H2SO4): 15 mL
6. Sample diluent (PBS) 2x
7. Washing Solution (PBS + Tween 20): 60 mL as 10x
8. Two plastic foils to cover the strips during the incubation
9. Instruction Manual

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength

Gluten ELISA Kit Background Information:
Gluten is the main part of the protein fraction of cereals and consists of nearly the equal amount of the protein compounds prolamin (gliadin) and glutenin. Because of its special physico-chemical attributes and its low price, gluten is not only contained in cereal products, but also in other food as sausage products and ice cream or in drugs and cosmetics as binder and filler.
For some persons, gluten has a pathological effect (coeliac disease). These people need to have a strict gluten free diet. In the European Union a maximum level of 20 ppm gluten is allowed for products declared as a gluten-free, and 100 ppm gluten for products declared as a very low gluten respectively. Sensitive detection systems are required to determine gluten residues in foodstuff.

Gluten ELISA Kit Test Principle:
The DAI Gliadin/Gluten quantitative test is based on the principle of the enzyme-linked immunosorbent assay. An antibody directed against gliadin (the soluble fraction of gluten) is bound on the surface of a microtiter plate.

Diagnostic Automation Inc. offers several popular Food ELISA Test Kits: Streptomycin ELISA Kit, Aflatoxin M1 ELISA Kit, Penicillin ELISA Kit, Histamine ELISA Kit, and Verotoxin Antigen in Food ELISA Test Kit. For more information on any of these Food ELISA Test Kits please see our website home page, or contact our Customer Service Representative at 818-591-3030.

Product Note:

Diagnostic Automstion, Inc. limit of detection (LOD) of the DAI Gliadin/Gluten test is 0.3 ppm Gliadin.

The limit of quantification (LOQ) of the DAI Gliadin/Gluten test is 2 ppm Gliadin. Due to the variety of sample matrices and their influence on the blank, results less than the LOQ should be treated as negative. This DAI test has been in big demand in the recent years and have been sold to many countries