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Human HBeAb ELISA Test Kit, Export Use Only

Category Name Blood bank ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle Competitive ELISA
Detection Range Qualitative Positive; Negative control & Cut off
Sample 50ul Serum
Specificity 99.7 %
Sensitivity 100 %
Total Time ~ 75 min
Shelf Life 12-18 Months from the manufacturing date

Item #:                    1706-12   Quantity:               




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HBeAb ELISA kit description:

"Export Use Only"
This anti-HBe ELISA test is an enzyme-linked immunosorbent assay for the qualitative identification of antibodies to hepatitis B virus e antigen (anti-HBe) in human serum/plasma.The purpose of the HBeAb ELISA Test is for clinical lab diagnosis of patients who are suspected of having a hepatitis B virus infection.

Materials included with HBeAb ELISA test kit:
1. Microwell Plate: 96 wells coated with recombinant HBeAg
2. Negative Control: Protein-stabilized buffer non-reactive for anti-HBe
3. Positive Control: anti-HBe diluted in protein-stabilized buffer
4. HRP-Conjugated Reagent: HRP-conjugated purified anti-HBe
5. Stock Wash Buffer: PH 7.4, 20x PBS
6. Chromogen Solution A: Urea peroxide solution
7. Chromogen Solution B: TMB solution
8. Stop Solution: Diluted sulfuric acid solution
9. Plastic Sealable Bag
10. Cardboard Plate Cover
11. Package Insert

Materials Required, not Provided:
1. Dispensing system and/or Precision pipettes
2. Distilled or deionized water
3. Microshaker for dissolving and mixing conjugate with samples
4. EIA kit Microplate Washer
5. EIA kit Microplate Reader with 450nm or dual wavelength 450 & 630nm

HBeAb ELISA test Background Information:
Acute phase viral infection is directly related to the presence of e-antigen. Acute hepatitis B recovery after treatment involves the first serological marker, HBeAg negative. It would be replaced by the corresponding antibody (anti-HBe). When using HBeAg as a specific marker of infection, the actual presence of anti-HBe antibody in blood is then considered an indication of recovery from infection. Even if HBeAg is not detectable, acute HBV infections can occur and this usually indicates presence of HBV mutants. Any phase of infection in these cases may signal high, low, or non-detectable quantities of HBV. Having an HBeAb ELISA for these patients is important in order to monitor the progress of HBV.

HBeAb ELISA test Principle:
The HBeAb ELISA Test kit uses the principle of solid phase, one-step incubation, competitive ELISA. If anti-HBe is present, it competes with purified anti-HBe conjugated to horseradish peroxidase for a set number of purified HBeAg pre-coated in the wells.

Most infectious disease ELISA kits follow a similar method. See the HCV Ab ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.

For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.

*Not to be sold, shipped, or distributed in The United States.

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