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HIV 1,2 ELISA kit


HIV 1,2 ELISA kit

Full name

Human Immunodeficiency Viruses Type 1,2 Antibody (HIV1,2) ELISA Test Kit (CE certified)

Category Name Infectious Disease ELISA kits
Test 96,192,480
Method ELISA: Enzyme Linked Immunosorbent Assay
Principle ELISA: Sandwich; Double antigen
Detection Range Qualitative: Positive, negative and cutoff calibrators
Sample 100 uL Serum or plasma
Specificity 99.89%
Sensitivity 99.95%
Total Time ~75 Min
Shelf Life 12-13 Months from the manufacturing date

Item #:                    1516-12   Quantity:               

HIV 1,2 ELISA kit


HIV 1,2 ELISA kit

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HIV 1,2 ELISA kit description:

Diagnostic Automation offers a HIV 1, 2 ELISA kit is an enzyme-linked immunosorbent assay for the qualitative detection of antibodies to HIV type 1 (group M-O) or type 2 in human serum or plasma samples. The HIV 1, 2 ELISA assay Kit is designed as an aid for screening of blood donors and/or as an aid in diagnosis of clinical conditions related to infection with HIV-1 and HIV-2, the etiological agents of AIDS. The Sensitivity of the HIV 1, 2 ELISA kit is 100% and the Specificity is 99.89%.

Materials Provided with HIV 1/2 Elisa (EIA)kits

1. Microwell strips breakable: 8 X 12/12 X 8-well strips per plate, contains recombinant HIV 1/2 antigens.
2. HIV 1/2 Elisa test Negative Control: 1vial
Yellowish liquid filled in a vial with green cap
3. HIV 1/2 Elisa test Positive Control Serum-1 (HIV 1): 1vial
Red-colored liquid in a red cap vial, 1ml
Antibodies to HIV 1 diluted in protein-stabilized buffer
4. HIV 1/2 Elisa test Positive Control Serum-2 (HIV 2): 1vial
Red-colored liquid yellow cap vial, 1ml
Antibodies to HIV 2 diluted in protein-stabilized buffer
5. HIV 1/2 EIA HRP-Conjugate Reagent: 13 ml per vial
Red-colored liquid in a white vial with red/orange cap
6. Wash Buffer 20 x: 50 ml
Colorless liquid in a clear bottle with white screw cap
7. Chromogen Solution A HIV 1/2 EIA Test: 8ml
Colorless liquid in a white vial with green cap
8. Chromogen Solution B HIV 1/2 EIA Test: 8ml
Colorless liquid in a brown vial with brown/black cap
9. Stop Solution: 8 ml
Colorless liquid in a white vial with yellow cap
10. Plastic sealable Bag, 1unit
11. Cardboard Plate Cover, 2sheets
12. Package Inserts

Materials & Instrumentations Required not provided
1. Freshly distilled or deionized water
2. Disposable gloves and timer
3. Appropriate waste containers for potentially contaminated materials
4. Dispensing system and/or pipette & tips
5. Dry incubator or water bath
6. ELISA Microshaker for dissolving and mixing conjugate with samples
7. EIA kit Microplate Washer
8. EIA kit Microplate Reader at 450nm wavelength

HIV 1/2 Elisa/EIA kit Background Information
HIV-1 was thought to be the only cause of these syndromes until 1986, when a second type of Human Immunodeficiency Virus (HIV-2) was isolated and also reported to cause AIDS (acquired immunodeficiency syndrome). Both viruses have the same morphology and lymphotropism, and the modes of transmission appear to be identical. In addition, HIV-1 and HIV-2 genomes exhibit about 60% homology in conserved genes such as gag and pol. Serologic studies have also shown that the core proteins of HIV-1 and HIV-2 display frequent cross-reactivity whereas the envelope proteins are more type-specific. Despite this immunologic cross-reactivity, detection of antibodies to HIV-2 with any of the licensed HIV-1 enzyme immunoassays is highly variable. This HIV1/HIV-2 ELISA kit was developed to detect antibodies to HIV-1 and /or HIV-2, for blood screening and diagnostic purposes. Testing for the presence of HIV antigens or antibodies from serum brings serological evidence of infection from individuals that may be harboring the HIV virus. Detection of antigens can be evidenced in both the acute phase and symptomatic phases of AIDS. However, the HIV-1 and HIV-2 antibodies can be identified all through the entire infection period - beginning at the acute phase all the way through the last stages of AIDS. For the serodiagnosis of HIV infection, the best approach is use of highly sensitive antibody ELISA tests. The predominant path of HIV infection is blood transfusion (independent of the other dominant carrier - sexual transmission). All donated blood or plasma is tested because HIV can be present in both cellular and cell-free fractions of human blood. This creates a high risk transmission rate if not tested properly. Thus, HIV ELISA kits can be an important screening tool.

HIV 1/2 Elisa Assay Kit Principle
Polystyrene microwell strips pre-coated with recombinant HIV antigens indicated make up this two-step incubation antigen sandwich enzyme immunoassay kit (HIV1, 2 ELISA). In the first incubation step, the patient's plasma or serum sample is added. If specific HIV1/2 antibodies are present, they will be captured inside the wells. Afterwards, microwells are washed to remove unbound serum proteins. After this stage the second set of recombinant antigens conjugated to the enzyme Horseradish Peroxidase (HRP-Conjugate) is added. While the second incubation occurs, these antigens will bind to the captured antibody. The chromogen solution is added to the wells, after wash (to remove unbound conjugate). In the wells where the antigen-antibody-antigen (HRP) sandwich immunocomplex is taking place, the colorless chromogens are hydrolyzed by the bound HRP conjugate to a blue-colored product. After, the reaction is stopped and the blue color turns yellow.

HIV 1/2 Elisa Test Assay Kit Performance Characteristics
The clinical performances of this HIV 1+2 ELISA have been evaluated by a panel
of samples obtained from 11045 healthy blood donors in 10 testing centers and by a
panel of samples from 783 HIV 1/2 positive patients -757 HIV -1, 7 HIV-2, 2 HIV-O
and 17 HIV-1(M). The positive samples have been characterized based upon the
individual patient CD4+ count and/or Western Blot (WB) or NAT.
Analytical specificity
No cross reactivity was observed with specimens from patients infected with HAV,
HCV, HBV, HTLV, CMV, and TP. No high dose hook effect and rheumatoid factor
interference observed during clinical testing. The assay performance characteristics
are unaffected from elevated concentrations of bilirubin, hemoglobin, and triolein.

HIV 1/2 Elisa Related Kits:

HIV 1,2 Ab/Ag ELISA kit Cat # 1520-12


HIV 1/2 Rapid Test (Serum, Plasma,WB) Cat # 177575-1-12


Diagnostic Automation Inc. also provides other Test Kits:


For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.

Product Note:

Diagnostic Automation/Cortez Diagnostics  HIV 1, 2 ELISA Test kit is an enzyme-linked immunosorbent assay which is used for the qualitative detection of antibodies to HIV (human immunodeficiency virus) type 1 (group M-O) or type 2 in human serum/plasma.

Both HIV-1 and HIV-2 types are transmitted by sexual contact, through blood, and from mother to child to cause AIDS. HIV stands for human immunodeficiency virus. It is the virus that can lead to acquired immunodeficiency syndrome called AIDS.

HIV has few or no symptoms for up to 10 years or sometimes even more before symptoms of AIDS develop so it is crucial to be tested as soon as possible.

Diagnostic Automation, Inc. HIV 1,2 ELSA test kit includes ready to use reagents, small sample volume of 100 ul with 100% sensitivity.No cross reactivity with specimens from patients infected with HAV, HCV, HBV, HIV, CMV, and TP. No interference is observed from rheumatoid factors up to 2000U/ml. No high dose hook effect observed during clinical testing.

Our HIV test kit enables medical field professionals to identify HIV infection earlier and enabling the infected individuals to seek treatment sooner. Our HIV ELISA has been the best test as a diagnostic aid in detecting HIV-1 and HIV-2 infections, also has been used for blood donor screening and for HIV infection control and prevention programs globally in many countries. You may find more information about our HIV test in our website under Infectious disease/HIV ELISA test category.

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