HSV 1 IgM Chemiluminescence ELISA is intended for the detection of IgM antibodies to herpes simplex
virus (HSV) 1.
Herpes Simplex Virus is a common pathogen and its primary infection is usually asymptomatic. There are
two immunologically distinct types of HSV: Type 1 and Type 2. HSV 1 is generally associated with oral
infection and lesions above the waist, and HSV 2 is associated with genital infections and lesions below the waist. Clinical cases primarily are 1) eczema herpeticum with eczematous skin changes with numerous lesions, 2) Gingivo-stomatitis and 3) Herpes sepsis, almost only found in newly born of premature infants. HSV IgM Chemiluminescence ELISA is an accurate serologic method to detect HSV specific antibody IgM in serum sample.
Purified HSV antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and
the HSV 1 IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and substrate A & substrate B mixture is added. The light generated (RLU) is proportional to the amount of IgM specific antibody in the sample. The results are read by a microwell luminometer compared in a parallel manner with calibrator and controls.