HAV IgM ELISA kit description:

HAV ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for qualitative determination of IgM-class antibodies to hepatitis A virus in human serum or plasma. It is intended for use in clinical laboratories for diagnosis of acute hepatitis A and managements of patients related to infection with hepatitis A virus.
HAV ELISA kit is a solid phase, two-step incubation, antibody capture ELISA assay in which, polystyrene microwell strips are pre-coated with antibodies directed to human immunoglobulin M proteins (anti-μ chain). The patient’s serum/plasma sample is added and during the first incubation, any IgM antibodies will be captured in the wells. After washing out all the other components of the sample and in particular IgG antibodies, the specific HAV IgM captured on the solid phase is detected by the addition of HAV antigens conjugated to horseradish peroxidase (HRP-Conjugate). During the second incubation, the HRP-conjugated antigens will specifically react only with the HAV IgM antibodies and after washing to remove unbound HRP-conjugate, Chromogen solutions are added to the wells.

In presence of the (anti-μ)-(HAV-IgM)-(antigen-HRP) immunocomplex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color intensity can be measured and is proportional to the amount of antibody captured in the wells, and to the sample respectively. Wells containing samples negative for HAV-IgM remain colorless.

The infection with HAV induces strong immunological response and elevated levels first of IgM and then IgG are detectable within a few days after the onset of the symptoms. The presence of anti–HAV IgM is an important serological marker for early detection and observation of the clinical manifestation of the disease. Increasing levels of anti-HAV IgM are detectable about three weeks after exposure with highest titter after four to six weeks later. Within six months after infection IgM concentration declines to non-detectable levels.*

* Not to be sold, shipped or distributed in The United States.