Herpes Simplex 1 IgG (HSV-1 IgG) ELISA kit description:

The DIAGNOSTIC AUTOMATION ELISA, HSV 1 IgG is intended for use in evaluating a patient's serologic status to herpes simples virus (HSV) infection, or for evaluating paired sera for the presence of a significant increase in herpes specific IgG.

Herpes Simplex Virus is a common pathogen and its primary infection is usually asymptomatic. There
are two immunologically distinct types of HSV: Type 1 and Type 2. HSV 1 is generally associated with
oral infection and lesions above the waist, and HSV 2 is associated with genital infections and lesions
below the waist. Clinical cases primarily are 1) eczema herpeticum with eczematous skin changes with
numerous lesions, 2) Gingivo-stomatitis and 3) Herpes sepsis, almost only found in newly born of premature infants. DIAGNOSTIC AUTOMATION ELISA HSV IgG is an accurate serologic method to detect HSV specific antibody in serum sample.

Purified HSV antigen is coated on the surface of microwells. Diluted patient serum is added to wells,
and the HSV 1 IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and TMB Chromogenic substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.