Herpes Simplex 2 IgA (HSV-2 IgA) ELISA kit
|Category Name||Infectious Disease ELISA kits|
|Method||ELISA method: Enzyme Linked Immunosorbent Assay|
|Principle||ELISA principle- Indirect; Antigen Coated Plate|
|Detection Range||Qualitative elisa assay- Positive, Negative and Cut-off|
The DIAGNOSTIC AUTOMATION ELISA, HSV 2 IgA is intended for the detection of IgA antibodies to herpes simplex virus 2 (HSV 2).
Purified HSV antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the HSV 2 IgA specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and TMB Chromogenic substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgA specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.
Herpes Simplex Virus is a common pathogen and its primary infection is usually asymptomatic. There are two immunologically distinct types of HSV: Type 1 and Type 2. HSV 1 is generally associated with oral infection and lesions above the waist, and HSV 2 is associated with genital infections and lesions below the waist. Clinical cases primarily are 1) eczema herpeticum with eczematous skin changes with numerous lesions, 2) Gingivo-stomatitis and 3) Herpes sepsis, almost only found in newly born of premature infants. The antibodies to HSV 2 may be of the IgA, IgM and IgG isotypes.