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Human Mycobacterium Tuberculosis (TB) IgM ELISA Test

Name

Mycobacterium Tuberculosis (TB) IgM ELISA kit

Full name

Human Mycobacterium Tuberculosis (TB) IgM ELISA Test kit

Category Name Infectious Disease ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA principle- Indirect; Antigen Coated Plate
Detection Range Quantitative elisa assay- Positive, Negative and Cut-off
Sample 5ul
Specificity 100%
Sensitivity 100%
Total Time ~110 min
Shelf Life 12 Months from the manufacturing date

Item #:                    5112-8   Quantity:               

Human Mycobacterium Tuberculosis (TB) IgM ELISA Test


Human Mycobacterium Tuberculosis (TB) IgM ELISA Test

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Human Mycobacterium Tuberculosis (TB) IgM ELISA Test description:




Diagnostic Automation Inc. Mycobacterium Tuberculosis IgM ELISA Kit is an Enzyme immunoassay (EIA) for the detection and quantitative determination of human IgM antibodies against Mycobacterium Tuberculosis in human serum and plasma. Further applications of this elisa kit in other body fluids are possible and can be requested from our Technical Service at 818-591-3030.





Materials Provided with Mycobacterium Tuberculosis IgM ELISA Test Kit:
1. Mycobacterium Tuberculosis IgM EIA Kit Microtiter Strips: wells, coated with a M. tuberculosis antigen mixture(recombinant Mycobacterium tuberculosis antigens
2. Mycobacterium Tuberculosis IgM ELISA Kit Calibrator A (Negative Control)No IgM
3. Mycobacterium Tuberculosis IgM ELISA Kit Calibrator B (Cut-Off Standard)
low concentration of IgM antibodies against Mycobacterium tuberculosis
4. Mycobacterium Tuberculosis IgM ELISA Kit Calibrator C (Weak Positive Control), medium concentration of IgM antibodies against Mycobacterium tuberculosis
5. Mycobacterium Tuberculosis IgM ELISA Test Kit Calibrator D (Positive Control), 2 ml
high concentration of IgM antibodies against Mycobacterium tuberculosis
6. Influenza A IgM Eia Test Enzyme Conjugate,
anti-human-IgM-HRP (rabbit)
7. Mycobacterium Tuberculosis IgM Eia Test Kit
8. Stop Solution
9. Sample Diluent
10. Mycobacterium Tuberculosis IgM Test Washing Buffer Washing Buffer
11. Plastic Foils
12. Plastic Bag

Materials & Instrumentations required not included:
1. Reagent tubes for the serum dilution
2. Bidistilled water
3. Precision pipettes, 5-100 & 500 ul
4. EIA Kit Microplate Washer
5. EIA Kit Microplate Reader at 450 nm




Mycobacterium Tuberculosis IgM ELISA (EIA) Kit Background Information
Mycobacterioses (tuberculosis, leprosy, atypical mycobacterioses, paratuberculosis, and perhaps Crohn Disease) are the infectious diseases of men and animals with the largest diffusion on earth. The infectious agents of tuberculosis are acid-resistant rod-like formed bacteria of the family Mycobacteriaceae, genus Mycobacterium. The germ was detected by Robert Koch in 1882. Owing to the very high infectious power of pathogenic mycobacteria, early diagnosis is essential to prevent spreading of the disease. Convergence of various approaches is necessary to control the mycobacterioses, immune reactions and bacterial shedding being variable during the diseases. However, usual diagnostic procedures were up to now unsatisfactory and did not allow distinguishing among different mycobacterial species. The illness is normally transferred by droplets of saliva from infected persons. This ELISA test kit for the determination of IgM antibodies uses a cocktail of highly pure proteins in order to determine an immune response against the bacteria in human serum. A fresh or chronically active infection can be diagnosed by IgA and IgG tests, which are also available.




Mycobacterium Tuberculosis IgM ELISA (EIA) Test Principle
Mycobacterium tuberculosis antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgM antibodies of the serum and the immobilized Mycobacterium tuberculosis antigen takes place. After one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgM peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of IgM antibodies is directly proportional to the intensity of the color.





Precautions on Mycobacterium Tuberculosis IgM ELISA (EIA) Kit
All sera and plasma or buffers based upon, have been tested respective to HBsAg, HIV and HCV with recognized methods and were found negative. Nevertheless precautions like the use of latex gloves have to be taken.



Mycobacterium Tuberculosis IgM ELISA Related Kits:


Mycobacterium Tuberculosis IgA ELISA Kit

http://www.rapidtest.com/index.php?i=Mycobacterium-Tuberculosis-IgA-(TB)-ELISA-Test&id=99&cat=16

Mycobacterium Tuberculosis IgG ELISA Kit

http://www.rapidtest.com/index.php?i=-Mycobacterium-Tuberculosis-IgG-(TB)-IgG-ELISA-kit&id=100&cat=16



Diagnostic Automation Inc. also offers other Test Kits:

http://www.rapidtest.com/index.php?product=Rapid-Tests

For more information about ELISA Kits, Rapid Tests, CLIA tests, or IFA Tests, please contact our Customer Service Representatives at 818-591-3030.







Product Note:

Our TB IgM ELISA Kit have high sensitivity and specificity.