Cytomegalovirus IgM (CMV IgM) ELISA kit
|Category Name||Infectious Disease ELISA kits|
|Method||ELISA method: Enzyme Linked Immunosorbent Assay|
|Principle||ELISA principle- Indirect; Antigen Coated Plate|
|Detection Range||Qualitative elisa assay- Positive, Negative and Cut-off|
|Total Time||~75 min|
The DIAGNOSTIC AUTOMATION ELISA, CMV IgM is intended for use in the detection of IgM antibodies to Cytomegalovirus (CMV) infection. Cytomegalovirus is a herpes virus and a leading biological factor causing congenital abnormalities and complications among those who receive massive blood transfusions and immunosuppressive therapy. About half of pregnant women who contract a primary infection spread the disease to their fetus. When acquired in utero, the infection may cause mental retardation, blindness, and/or deafness. Serological tests for detecting the presence of antibody to CMV can provide valuable information regarding the history of previous infection, diagnosis of active or recent infection, as well as in screening blood for transfusions in newborns and immuno-compromised recipients. DIAGNOSTIC AUTOMATION ELISA CMV IgM is an accurate serologic method to detect CMV IgM antibody for identification of CMV infection. Purified CMV antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the CMV IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and TMB Chromogenic Substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgM specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.