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HSV-1 IgA ELISA kit

Name

HSV-1 IgA ELISA kit

Full name

Human Herpes Simplex Virus1 IgA (HSV-1 IgA) ELISA Test kit

Category Name Infectious Disease ELISA kits
Test 96
Method ELISA: Enzyme Linked Immunosorbent Assay
Principle ELISA - Indirect; Antigen Coated Plate
Detection Range Qualitative - Positive, Negative and Cut-off
Sample 5ul
Specificity 98%
Sensitivity 98%
Total Time 90min
Shelf Life 12-18months

Item #:                    1400-11   Quantity:               

HSV-1 IgA ELISA kit

HSV 1 IgA ELISA MSDS (02-02-2017).pdf

HSV-1 IgA ELISA kit

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HSV-1 IgA ELISA kit description:




Diagnostic Automation HSV1 IgA elisa test kit is intended for use in evaluating a patient's
serologic status to Herpes Simplex Virus (HSV) infection, or for evaluating paired sera for the presence of a significant increase in herpes specific IgA.




Material Provided with HSV-1 IgA ELISA Kit:
1. HSV1 IgA EIA Test Microwell Strips: Purified HSV1
antigen coated wells
2. Absorbent Solution
3. Calibrator: Factor value (f)
4. HSV1 IgA Elisa kit Negative Control
5. HSV1 IgA Elisa kit Positive Control
6. Washing Concentrate 10X 1 bottle
7. Herpes Simplex Virus1 IgA Test Enzyme Conjugate
8. TMB Chromogenic Substrate
9. HSV1 IgA EIA Kit Stop Solution

Materials & Instrumentations required not included:
1. Deionized/distilled water
2. EIA Kit Microplate Washer
3. EIA Kit Microplate Reader at 450





HSV1 IgA elisa test kit Background Information
Herpes Simplex Virus (HSV) also known as Human herpes virus is a member of the herpes virus family, Herpesviridae. HSV is a common pathogen and its primary infection is usually asymptomatic. There are two immunologically distinct types of HSV: Type 1 and Type 2. HSV 1 is generally associated with oral infection and lesions above the waist, and HSV 2 is associated with genital infections and lesions below the waist. Clinical cases primarily are 1) eczema herpeticum with eczematous skin changes with numerous lesions, 2) Gingivo-stomatitis and 3) Herpes sepsis, almost only found in newly born of premature infants. HSV1 IgA elisa test kit is an accurate serologic method to detect HSV specific antibody in serum sample.




HSV1 IgA elisa test kit Principle
Purified HSV antigen is coated on the surface of microwells. Diluted patient serum is added to wells. HSV 1 IgA specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and TMB Chromogenic substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgA specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.



HSV1 IgA elisa test kit Performance and Characteristics

HSV1 IgA elisa test Results Interpretation
Negative: HSV1 A Index less than 0.90 are negative for IgA antibody to HSV1
Equivocal: HSV1 A Index between 0.91-0.99. Sample should be retested
Positive: HSV1 A Index of 1.00 or greater are positive for IgA antibody to HSV1



HSV1 IgA elisa test kit Performance & Characteristics
Precision
The precision of the assay was evaluated by testing three different sera eight replicates over a period of one week. The intra-assay and inter-assay C.V. are:
Intra-assay: Negative 9.8% Low positive 7.5% Positive 5.6%
Inter-assay: Negative 10.8% Low positive 8.9% Positive 6.7%




HSV1 IgA elisa test Related Kits:

HSV1 IgG elisa test kit

http://www.rapidtest.com/index.php?i=Herpes-Simplex-1-IgG-(HSV-1-IgG)-ELISA-kit&id=111&cat=16

HSV1 IgM elisa test kit

http://www.rapidtest.com/index.php?i=Herpes-Simplex-1-IgM-(HSV-1-IgM)-ELISA-kit&id=112&cat=16


HSV-1-2 IgG ELISA kit

http://www.rapidtest.com/index.php?i=Herpes-Simplex-1,2-IgG-(HSV-1-2-IgG)-ELISA-kit&id=117&cat=16

HSV-1-2 IgM ELISA kit

http://www.rapidtest.com/index.php?i=Herpes-Simplex-1,2-IgM-(HSV-1-2-IgM)-ELISA-kit&id=118&cat=16


HSV2 IgA ELISA kit

http://www.rapidtest.com/index.php?i=Herpes-Simplex-2-IgA-(HSV-2-IgA)-ELISA-kit&id=116&cat=16


HSV2 IgG ELISA kit

http://www.rapidtest.com/index.php?i=Herpes-Simplex-2-IgG-(HSV-2-IgG)-ELISA-kit&id=114&cat=16


HSV2 IgM ELISA kit

http://www.rapidtest.com/index.php?i=Herpes-Simplex-2-IgM-(HSV-2-IgM)-ELISA-kit&id=115&cat=16


HSV1 IgA elisa test Related IFA (Immunofluorescence Assay) Kits:

HSV1 IgG IFA Kit

http://www.rapidtest.com/index.php?i=HSV-1-IgG-IFA-Kit---(Immunofluorescence-Assay)-&id=507&cat=36


HSV1 IgM IFA Kit

http://www.rapidtest.com/index.php?i=HSV-1-IgM-IFA-Kit---(Immunofluorescence-Assay)-&id=508&cat=36


HSV2 IgG IFA Kit
http://www.rapidtest.com/index.php?i=HSV-2-IgG-IFA-Kit---(Immunofluorescence-Assay)-&id=511&cat=36

HSV2 IgM IFA Kit

http://www.rapidtest.com/index.php?i=HSV-2-IgM-IFA-Kit---(Immunofluorescence-Assay)-&id=512&cat=36


HSV1 IgA elisa test Related Chemiluminescence Immuno Assay (CLIA) Kits:


HSV1 IgG CLIA Kit

http://www.rapidtest.com/index.php?i=HSV-1-IgG-CLIA-kits---(Chemiluminescence-Immuno-Assay)&id=546&cat=41


HSV1 IgM CLIA Kit

http://www.rapidtest.com/index.php?i=HSV-1-IgM-CLIA-kits---(Chemiluminescence-Immuno-Assay)&id=547&cat=41


HSV 2 IgG CLIA kits

http://www.rapidtest.com/index.php?i=HSV-2-IgG-CLIA-kits---(Chemiluminescence-Immuno-Assay)&id=548&cat=41


HSV 2 IgA CLIA kits

http://www.rapidtest.com/index.php?i=HSV-2-IgM-CLIA-kits---(Chemiluminescence-Immuno-Assay)&id=549&cat=41



Diagnostic Automation Inc. also provides other Test Kits:

http://www.rapidtest.com/index.php?product=Rapid-Tests


For more information about ELISA Kits, Rapid Tests, CLIA tests, or IFA Tests, please contact our Customer Service Representatives at 818-591-3030.


Product Note:

Herpes Simplex Virus is a common pathogen and its primary infection is usually asymptomatic. There are two immunologically distinct types of HSV: Type 1 and Type 2. HSV 1 is generally associated with oral infection and lesions above the waist, and HSV 2 is associated with genital infections and lesions below the waist. Clinical cases primarily are 1) eczema herpeticum with eczematous skin changes with numerous lesions, 2) Gingivo-stomatitis and 3) Herpes sepsis, almost only found in newly born of premature infants. The antibodies present to HSV 1 may be of the IgA, IgM and IgG isotypes. The physiological function of IgA and its clinical implication is still unclear.