Herpes Simplex 1,2 IgM (HSV-1-2 IgM) ELISA kit description:

The DIAGNOSTIC AUTOMATION ELISA, HSV 1,2 IgM is intended for the detection of IgM antibodies
to herpes simplex virus (HSV) 1 and 2.

Herpes Simplex Virus is a common pathogen and its primary infection is usually asymptomatic. There
are two immunologically distinct types of HSV: Type 1 and Type 2. HSV 1 is generally associated with
oral infection and lesions above the waist, and HSV 2 is associated with genital infections and lesions
below the waist. Clinical cases primarily are 1) eczema herpeticum with eczematous skin changes with
numerous lesions, 2) Gingivo-stomatitis and 3) Herpes sepsis, almost only found in newly born of premature infants. DIAGNOSTIC AUTOMATION ELISA HSV IgM is an accurate serologic method to
detect HSV specific antibody IgM in serum sample.

Purified HSV antigen is coated on the surface of microwells. Diluted patient serum is added to wells,
and the HSV IgM specific antibody, if present, binds to the antigen. All unbound materials are washed
away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and TMB Chromogenic substrate is added. The enzyme conjugate catalytic
reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount
of IgM specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.