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Mumps IgM ELISA kit

Name

Mumps IgM ELISA Test

Full name

Human Mumps IgM ELISA Test Kit

Category Name Infectious Disease ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle Indirect ELISA: Antigen Coated Plate
Detection Range Qualitative: Positive, Weak Positive, Negative Control
Sample 10 uL
Specificity 100%
Sensitivity 100%
Total Time ~75 min
Shelf Life 12 Months from the manufacturing date

Item #:                    1411-1   Quantity:               

Mumps IgM ELISA kit


Mumps IgM ELISA kit

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Mumps IgM ELISA kit description:




The Diagnostic Automation Mumps IgM ELISA kit is intended for the qualitative detection of Mumps IgM antibody in human serum.




Materials Provided with Mumps IgM ELISA Kit:
1. Microassay plate: 96 wells coated with purified Mumps antigen
2. Calibrator: Human serum or defibrinated plasma
3. Positive Control: Human serum or defibrinated plasma
4. Negative Control: Human serum or defibrinated plasma
5. HRP Conjugate: Ready to use, Goat anti-human IgM
6. Serum Diluent PlusI
7. Wash Buffer Type I, 20X
8. Chromogen/Substrate Solution Type I
9. Stop Solution: Ready to use,1N H2SO4 solution

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450 single & 600-650nm dual wavelength




Mumpss IgM ELISA Kit Background Information:
The mumps virus is a member of the paramyxovirus group and the etiological agent of mumps in man. Mumps is a generalized illness usually accompanied by parotid (salivary gland) swelling and mild symptoms. It is also one of the most common causes of aseptic meningitis, encephalitis, and inflammation of the testes (orchitis), pancreas, and ovaries. Current methods for serodiagnosis of mumps infections are in-vitro serum neutralization, hemagglutination-inhibition (HAI), indirect immunofluorescence, and complement fixation (CF) tests. Of these methods, neutralization is reportedly the most specific. However, the neutralization test requires 4-5 days to complete the test. HAI and CF are reportedly less sensitive than the neutralization test. These methods lack specificity, which limits their usefulness in determining immune status. The HAI test also requires pretreatment of test sera to remove nonspecific hemagglutination inhibitors from some sera. Anti-Mumps virus IgM appear 2-3 days after the occurrence of the first clinical symptoms (these remain 2-3 months), followed by the production of Mumps IgG antibodies which persist lifelong following vaccination with live virus there is a seroconversion in 90% of cases, however, the titre is somewhat lower than in normal infections. ELISA can be both specific and sensitive for the detection and measurement of serum proteins. The sensitivity, specificity, and reproducibility of elisa test can be comparable to other serological tests for antibody, such as immunofluorescence, complement fixation, hemagglutination and neutralization.



Mumps IgM ELISA Test Principle:
The Mumps test kit results are read by a microwell reader compared in a parallel manner with calibrator and controls. Diluted patient serum is added to wells, and the Mumps IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off, and TMB Chromogenic Substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgM specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.



For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.



Product Note:

DAI Mumps IgM Solid phase immunoassay for the detection of virus-specific IgM is known to be sensitive to interfering factors. This kit overcomes interference by treating samples prior to running the assay. The goat/sheep anti-human IgG in the Serum Diluent plus Solution diminishes competing virus-specific IgG, which would be responsible for false negative reactions. False positives are similarly minimized by removing the IgG, thus neutralizing the bound rheumatoid factor in the samples.