Lyme Disease ELISA kit description:

Los Angeles-based Diagnostic Automation/Cortez Diagnostics, Inc. offers a Lyme Disease ELISA Test, known as Lyme Disease IgG/IgM ELISA Test, which is a B. burgdorferi ELISA test system designed to detect IgM and IgG class antibodies to Borrelia burgdorferi in human sera.  The wells of the plastic micro well strips are sensitized by passive absorption with Borrelia burgdorferi antigen.  The Lyme Disease ELISA Test is for in-vitro diagnostic use only.

The Lyme Disease Test procedure involves three incubation steps:  (1)  Test sera (properly diluted) are incubated in antigen-coated micro wells of the Lyme Disease ELISA Test.  Any antigen specific antibody in the sample will bind to the immobilized antigen.  The plate is washed to remove unbound antibody and other serum components.  (2)  Peroxidase conjugated goat anti-human IgM/IgG is added to the wells of the Lyme Disease ELISA Test and the plate is then incubated.  The conjugate will react with IgM and/or IgG antibody immobilized on the solid phase in step 1.  The wells are washed to remove unreacted conjugate.  (3)  The micro wells of the Lyme Disease ELISA Test containing immobilized perioxidase conjugate are incubated with peroxidase substrate solution.  Hydrolysis of the substrate by peroxidase produces a color change.  After a period of time, the reaction is stopped and the color intensity of the solution is measured photometrically.  The color intensity of the solution on the Lyme Disease Test depends upon the antibody concentration in the original test sample.

It is recommended that specimen collection be carried out carefully.  No known test method can offer complete assurance that human blood samples will not transmit infection. Therefore, all blood derivatives should be considered potentially infectious.  Only freshly drawn and properly refrigerated sera obtained by approved aseptic venipuncture procedures should be used in this Lyme Disease Test.  No anticoagulants or preservatives should be added.  Avoid using hemolyzed, lipemic, or bacterially contaminated sera on this Lyme Disease ELISA Test.  Store sample at room temperature for no longer than 8 hours.  If testing is not performed within 8 hours, sera may be stored between 2 and 8 degrees Celsius for no longer than 48 hours.  If delay in testing is anticipated, store test sera at -20 degrees Celsius or lower.  When using this Lyme Disease ELISA Test, avoid multiple freeze/thaw cycles that may cause loss of antibody activity and give erroneous results.

A negative is indicated by no detectable antibody.  The result does not exclude B. burgdorferi infection.  An additional sample for this Lyme Disease ELISA Test should be tested within 4-6 weeks if early infection is suspected.  A positive indicates antibody to B. burgdorferi is detected.  See the Lyme Disease ELISA Test kit insert for more information about following specific procedures, establishing quality control, and calculating test results.

Diagnostic Automation/Cortez Diagnostics, Inc. also provides two other Lyme Disease ELISA Tests.  One test is the Lyme Disease IgM ELISA Test Kit which is an ELISA test system designed to detect IgM antibodies to B. burgdorferi in human sera.  The other test kit is a Lyme Disease IgG ELISA Test Kit which is designed to detect IgG class antibodies to B. burgdorferi in human sera.  For more information about these Lyme Disease ELISA Test Kits, please contact our Customer Service Department at 818-591-3030.