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Legionella IgG/IgM/IgA ELISA Test


Legionella IgG/IgM/IgA ELISA kit

Full name

Human Legionella pneumophila IgG/IgM/IgA Elisa Test Kit

Category Name Infectious Disease ELISA kits
Test 96
Method ELISA: Enzyme Linked Immunosorbent Assay
Principle Indirect ELISA: Antigen Coated Plate
Detection Range Qualitative Positive; Negative control & Cut off
Sample 10 uL
Specificity 93.1%
Sensitivity 92.3%
Total Time ~65 min
Shelf Life 12 Months from the manufacturing date

Item #:                    1651-P1   Quantity:               

Legionella IgG/IgM/IgA ELISA Test

Legionella IgG,A,M_1651-2 (MSDS) 05-18-2016.pdf

Legionella IgG/IgM/IgA ELISA Test

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Legionella IgG/IgM/IgA ELISA Test description:

The Diagnostic Automation Legionella ELISA test kit is an enzyme-linked immunosorbent assay for the qualitative detection of total antibody (IgG/IgM/IgA) to Legionella pneumophila serogroups 1-6 in human sera.

Materials Provided with Legionella IgG/IgM/IgA elisa kit:
1. Plate: wells coated with Formalin-inactivated preparation
of L. pneumophila group 1-6 antigens
2. HRP-Conjugate goat anti-human IgG/IgA/IgM
3. Positive Control: Monkey Serum
4. Calibrator: Monkey Serum
5. Negative Control: Human Serum
6. Sample Diluent
7. TMB
8. Stop Solution: containing 1M H2SO4, 0.7MHCI
9. Wash Buffer Concentrate 10X
10. Package Insert

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm

Legionella IgG/IgM/IgA elisa kit Background Information:
L. pneumophila displays a multitude of morphologies including the bacillus, coccobacillus, and elongated fusiform. Although often difficult to perform, the Gram stain will be Gram-negative. The antibody response to L. pneumophila may be both specific and nonspecific, since the patient may have antibodies to similar antigens from other Gram-negative bacteria. Optimum times for specimen collection appear to be within the first week of illness, or as soon as possible after the onset (acute specimen), and at least 3 weeks after the onset (convalescent specimen). By the IFA method, a single result of >1:256 is considered presumptive evidence of legionella infection. Diagnostic titers have been reported to be absent in as many as 25% of patients, but the use of multiple Legionella species as the antigen source and a polyvalent conjugate directed against IgG, IgM, and IgA maximize the accuracy of serological procedures.

Legionella IgG/IgM/IgA ELISA Test Principle:
Legionella IgG/IgA/IgM ELISA test kit is designed for Legionella pneumophila detection of antibodies in human sera. Wells of plastic microwell strips are sensitized by passive absorption with Legionella antigen. The test procedure involves three incubation steps. For additional details please refer to the instructions for use.

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For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 818-591-3030.

Product Note:

This Legionella IgG/IgM/IgA ELISA Kit have high sensitivity and sensitivity for in vitro diagnostic use.