Brucella IgM ELISA kit description:

The Diagnostic Automation Brucella IgM Antibody ELISA Test Kit has been designed for the the detection and the quantitative determination of specific IgM antibodies against Brucella in serum and plasma. Further applications in other body fluids are possible and can be requested from the Technical Service of Diagnostic Automation Inc. This assay is intended for in-vitro diagnostic use only.

Laboratory results can never be the only base of a medical report. The patient history and further tests have
additionally to be taken into account.


The Diagnostic Automation Brucella IgM antibody test kit is based on the principle of the enzyme immunoassay (EIA). Brucella antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgM antibodies of the serum and the immobilized Brucella antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready to-use anti-human-IgM peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgM antibodies is directly proportional to the intensity of the color.

Enzyme immunoassay based on microtiter plate for the detection and quantitative determination of human
IgA antibodies against Brucella in serum and plasma.