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HDV Ag ELISA kit

Name

HDV Ag ELISA Test

Full name

Human HDV Ag ELISA Test Kit, Export Use Only

Category Name Infectious Disease ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle Indirect Elisa: Antigen Coated Plate
Detection Range Qualitative: Positive & Negative Control
Sample 50ul
Specificity 100%
Sensitivity 100%
Total Time ~75 min
Shelf Life 12 Months from the manufacturing date

Item #:                    1868-12   Quantity:               

HDV Ag ELISA kit

HDV-Ag 1868-12 MSDS (05-18-2016).pdf

HDV Ag ELISA kit

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HDV Ag ELISA kit description:




"Export Use Only"
HDV-Ag ELISA kit, which is an enzyme-linked immunosorbent assay for the qualitative detection of hepatitis D antigens in human serum or plasma. The purpose of the HDV Ag ELISA Test is for clinical lab diagnosis of patients who are suspected of hepatitis D (HDV-Ag) virus infection, and for epidemic analysis.




Materials Provided with HDV Ag ELISA kit:
1. Microwell strips: purified HDV antibodies coated wells
2. Negative Control: non-reactive for HDV Ag
3. Positive Control: Purified HDV Ag diluted
4. HRP-Conjugated anti-HDV antibodies
5. Extraction Solution
6. Stock Wash Buffer: 20 x
7. Chromogen Solution A: Urea peroxide solution
8. Chromogen Solution B: TBM
9. Stop Solution
10. Plastic sealable Bag
11. Cardboard Plate Cover
12. Package Insert

Materials & Instrumentations Required, not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette & tips
3. Microshaker for dissolving and mixing conjugate with samples
4. EIA kit Microplate Washer
5. EIA kit Microplate Reader at 450nm wavelength




HDV Ag Elisa Kit Background Information:
What causes Hepatitis D virus is an agent known as Delta agent (a defective 36nm-43nm RNA virus) which puts it into the classification of Hepatitis delta virus. Hepatitis D can only reproduce when it is linked with Hepatitis B virus (HBV). The disease is transmitted through the skin or through sexual contact with infected blood. Generally, the relationship of HDV and HBV involves some of the most acute and chronic HBsAg carriers. Because of this co-infection, it is important to know whether the HDV and HBV developed at the same time, or if the patient was already a carrier of HBsAg. The co-infection, especially super-infected HDV, can develop into severe acute hepatitis disease. Chronically infected HBV patients infected with HDV have a 70-80% chance of developing cirrhosis of the liver. Serologically, it is important to know the specific HDV antibodies (anti-HDV) or antigens. The first antibody to appear at the acute stage is IgM anti-HDV, which is transient and quickly replaced by IgG anti-HDV. If the IgM anti-HDV infection persists, a chronic HDV infection develops and continues to reproduce. Through the course of HBV-HDV co-infection, and after the tenth week of exposure, identifiable concentrations of HDV antibodies appear. If cleared up during convalescence, then evidence of recovery is shown.




HDV Ag Elisa Test Principle:
The HDV-Ag ELISA employs the solid phase, two-step incubation double antibody sandwich method. Serum/plasma from patient is added together with extraction solution after the polystyrene microwell strips are pre-coated with purified antibodies specific to HDV. If the HDV virus is present, the HDV particles are disrupted and what's captured in the wells is the specific HDV antigens. At this stage, unbound serum proteins must be washed off the microwells.



Notable features of Infectious Disease ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Most Infectious Disease ELISA kits follow a similar method. See the HDV Ag ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.



Diagnostic Automation Inc provides other Infectious Disease ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.

* Not to be sold, shipped or distributed in The United States.



Product Note:

Either fresh serum or plasma samples can be used for Diagnostic Automation Inc. HDV Ag ELISA test kit. Blood collected by venipuncture should be allowed to clot naturally and completely. Care should be taken to ensure that the serum samples are clear and not contaminated by microorganisms. Any visible particulate matters in the sample should be removed by centrifugation at 3000 RPM (round per minutes) for 20 minutes at room temperature or by filtration on 0.22u filters. Plasma samples collected into EDTA, sodium citrate or heparin may be tested, but highly lipaemic, icteric, or hemolized samples should not be used as they can give false results in the assay. Do not heat inactivated samples. This can cause sample deterioration

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