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HAV IgM ELISA kit

Name

HAV IgM ELISA Test

Full name

Human HAV IgM ELISA Test Kit, Export Use Only

Category Name Infectious Disease ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle Competitive ELISA: Antibody Capture
Detection Range Qualitative Positive; Negative control & Cut off
Sample 100 ul serum/plasma
Specificity 99%
Sensitivity 100%
Total Time ~ 75 min
Shelf Life 12-18 Months from the manufacturing date

Item #:                    1718-12   Quantity:               

HAV IgM ELISA kit

HAV IgM_ELISA_1718-12(MSDS)(06-13-2013).pdf

HAV IgM ELISA kit

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HAV IgM ELISA kit description:




"Export Use Only"
The HAV IgM ELISA test is an enzyme-linked immunosorbent assay (ELISA) which is used for the qualitative determination of IgM-class antibodies to hepatitis A virus (HAV-IgM) in serum/plasma. The purpose of the HAV IgM ELISA Test is for diagnosing and monitoring of patients suspected of hepatitis A virus.




Materials Provided with HAV IgM ELISA Kit:
1. Microwell strips: anti-IgM antibodies coated wells
2. Negative Control: Protein-stabilized buffer
3. Positive Control: Purified anti-HAV IgM antibodies diluted
4. HRP Conjugate: HRP-HAV antigen
5. Stock Wash Buffer 20x
6. Chromogen Solution A: Urea peroxide solution.
7. Chromogen Solution B: TMB
8. Stop Solution
9. Plastic Sealable Bag
10. Cardboard Plate Cover

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength




HAV IgM ELISA Test Background Information:
Infections usually onset in early childhood, HAV can adversely affect the liver, but acute complications or chronic illness do not necessarily occur because Hepatitis A is a self-limited disease. Causes of the infection are connected to unsanitary conditions and poor hygiene within densely populated areas. Thus, the fecal-oral route of the disease is easily transmitted in this type of setting. One single infected person could cause an entire outbreak. Hepatitis A is a non-enveloped positive strand RNA virus with a linear single strand genome, encoded for only one known serotype. Within HAV are four major, structural polypeptides, concentrated exclusively in the cytoplasm of human hepatocites. An HAV infection causes a strong immunological response, and within a few days after the onset of symptoms, elevated levels of IgM, and subsequently IgG, are identified. The identification of anti-HAV IgM serves as a critical serological marker in detection and observation of how the disease has manifested in a clinical setting. After approximately three weeks of exposure, increasing levels of anti-HAV IgM are detectable, with the highest titter occurring after four to six weeks.





HAV IgM ELISA Test Principle:
The principle method of the HAV IgM ELISA is a solid phase two-step incubation, antibody capture ELISA assay. In this HAV IgM ELISA kit, polystyrene microwell strips are pre-coated with antibodies to human immunoglobulin M proteins (anti-u chain). During the first incubation, the serum or plasma sample is added. At this point, any IgM antibodies will be captured in the wells. Then HAV antigens conjugated to HRP-Conjugate is added, and any specific HAV IgM captured during the solid phase will be identified at this time. In the course of second incubation, the HRP-conjugated antigens will react only with the HAV IgM antibodies.



For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.

* Not to be sold, shipped or distributed in The United States.


Product Note:

The components of the Diagnostic Automation, Inc. ELISA kit will remain stable through the expiration date indicated on the label and package when stored between 2-8 C, do not freeze. To assure maximum performance of this HAV IgM ELISA kit, during storage protect the reagents from contamination with microorganism or chemicals.