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Human HDV IgG ELISA Test Kit, Export Use Only

Category Name Infectious Disease ELISA kits
Test 96
Method ELISA: Enzyme Linked Immunosorbent Assay
Principle Competitive ELISA
Detection Range Qualitative Positive; Negative control and Cut off
Sample 10ul Serum
Specificity 100%
Sensitivity 100%
Total Time ~ 75 min
Shelf Life 18 Months from the manufacturing date

Item #:                    1870-12   Quantity:               


MSDS-HDV IgG ELISA 1870-12 (MSDS)(05-18-2016).pdf


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HDV IgG ELISA kit description:

"Export Use Only"
The purpose of the HDV IgG ELISA Test is for clinical lab diagnosis and monitoring the treatment of patients who are suspected of having a hepatitis D virus infection. The HDV IgG ELISA test is an enzyme-linked immunosorbent assay for qualitative identification of IgG antibodies to hepatitis D virus in human serum/plasma.

Materials Provided with HDV IgG ELISA kit:
1. Microwell strips: purified HDV antigens coated wells
2. Negative Control: non-reactive for anti-HDV
3. Positive Control: Purified anti-HDV diluted
4. HRP-Conjugated anti-HDV antibodies
5. Specimen Diluent: Protein buffer solution
6. Stock Wash Buffer: 20 x
7. Chromogen Solution A: Urea peroxide solution
8. Chromogen Solution B: TBM
9. Stop Solution
10. Plastic sealable Bag
11. Cardboard Plate Cover
12. Package Insert

Materials & Instrumentations Required, not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette & tips
3. Microshaker for dissolving and mixing conjugate with samples
4. EIA kit Microplate Washer
5. EIA kit Microplate Reader at 450nm wavelength

HDV IgG Elisa Kit Background Information:
What causes Hepatitis D virus is an agent known as Delta agent (a defective 36nm-43nm RNA virus) which puts it into the classification of Hepatitis delta virus. Hepatitis D can only reproduce when it is linked with Hepatitis B virus (HBV). The disease is transmitted through the skin or through sexual contact with infected blood. Generally, the relationship of HDV and HBV involves some of the most acute and chronic HBsAg carriers. Because of this co-infection, it is important to know whether the HDV and HBV developed at the same time, or if the patient was already a carrier of HBsAg. The co-infection, especially super-infected HDV, can develop into severe acute hepatitis disease. Chronically infected HBV patients infected with HDV have a 70-80% chance of developing cirrhosis of the liver. Serologically, it is important to know the specific HDV antibodies (anti-HDV) or antigens. The first antibody to appear at the acute stage is IgM anti-HDV, which is transient and quickly replaced by IgG anti-HDV. If the IgM anti-HDV infection persists, a chronic HDV infection develops and continues to reproduce. Through the course of HBV-HDV co-infection, and after the tenth week of exposure, identifiable concentrations of HDV antibodies appear. If cleared up during convalescence, then evidence of recovery is shown.

HDV IgG Elisa Test Principle:
The HDV IgG ELISA kit employs the solid phase, two-step incubation, indirect ELISA method.
Recombinant HDV antigens are pre-coated on the polystyrene microwell strips. Anti-HDV specific antibodies, if present, will appear during the first incubation stage - and be bound to the solid phase precoated HDV antigens. After this step, the wells are washed to remove unbound serum proteins. Next it is important to add anti-human IgG antibodies (anti-IgG) conjugated to the enzyme HRP-Conjugate. These HRP-conjugated antibodies, during the second incubation stage, will be bound to any antigen-antibody (IgG) complexes that were formed before

Notable features of Infectious Disease ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Most Infectious Disease ELISA kits follow a similar method. See the HDV IgM ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.

Diagnostic Automation Inc provides other Infectious Disease ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.

* Not to be sold, shipped or distributed in The United States.

Product Note:

Non-repeatable positive result may occur due to the general biological and biochemical characteristics

of ELISA assays. The Diagnostic Automation, Inc. ELISA HDV IgG kit test is designed to achieve very high performance characteristics of sensitivity and specificity. However, antibodies may be undetectable during the early stages of the disease and in some immunosuppressed individuals.  Any positive results must be interpreted in conjunction with patient clinical information and other laboratory testing results.