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Pregnancy Rapid tests

RSV IgG ELISA kit

Name

RSV IgG ELISA kit

Category Name Infectious Disease ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA principle- Indirect; Antigen Coated Plate
Detection Range Qualitative elisa assay - Positive, Negative and Cut-off
Sample 100ul
Specificity 100%
Sensitivity 100%
Total Time 110min
Shelf Life 12-18months

Item #:                    5114-8   Quantity:               

RSV IgG ELISA kit

 

RSV IgG ELISA kit

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RSV IgG ELISA kit description:




Material Provided with RSV IgG ELISA Kit:

1.RSV IgG Kit Microtiter Strips: 12 strips with 8 breakable wells each, coated with a RSV antigen (purified RSV F+G antigen)
2.Calibrator A (Negative Control): 2 mL, protein solution diluted with PBS, contains no IgG antibodies against RSV. Addition of 0.01% methylisothiazolone and 0.01% bromonitrodioxane
3.Calibrator B (Cut-Off Standard): 2 mL human serum diluted with PBS, contains a low concentration of IgG antibodies against RSV. Addition of 0.01 % methylisothiazolone and 0.01 % bromonitrodioxane
4.RSV IgG Test Calibrator C (Weak Positive Control): 2 mL, human serum diluted with PBS, contains a medium concentration of IgG antibodies against RSV. Addition of 0.01 % methylisothiazolone and 0.01 % bromonitrodioxane
5.Calibrator D (Positive Control): 2 mL, human serum diluted with PBS, contains a high concentration of IgG antibodies against RSV. Addition of 0.01% methylisothiazolone and 0.01% bromonitrodioxane
6.Enzyme Conjugate: 15 mL, anti-human-IgG-HRP (rabbit), in protein-containing buffer solution. Addition of 0.01 % methylisothiazolone and 0.01 % bromonitrodioxane and 5 mg/L ProclinTM
7.RSV IgG Elisa kit Substrate: 15 mL, TMB (tetramethylbenzidine)
8.Stop Solution: 15 mL, 0.5 M sulfuric acid
9.Sample Diluent: 60 mL, PBS/BSA buffer. Addition of 0.095 % sodium azide
10.RSV IgG kit Washing Buffer: 60 mL, PBS + Tween 20, 10x concentrate. Final concentration: dilute 1+9 with distilled water. If during the cold storage crystals precipitate, the concentrate should be warmed up at 37C for 15 minutes
11.Plastic Foils: 2 pieces to cover the microtiter strips during the incubation
12.Plastic Bag: Resealable, for the dry storage of non-used strips

Materials & Instrumentations required but not included:

1.Reagent tubes for the serum dilution
2. Bidistilled water
3.Precision pipettes: 5 uL-, 100 uL- and 500 uL micro- and multichannel pipettes
4.EIA Kit Microplate Washer
5.EIA Kit Microplate Reader at 450

The Diagnostic Automation RSV IgG Antibody ELISA Test Kit has been designed for the the
detection and the quantitative determination of specific IgG antibodies against Respiratory syncytial
virus in serum and plasma. Further applications in other body fluids are possible and can be requested from the Technical Service of Diagnostic Automation. This assay is intended for in-vitro diagnostic use only. Laboratory results can never be the only base of a medical report. The patient history and further
tests have additionally to be taken into account.

Each year during the winter months, RS viruses spread heavily amongst children and infants. There
are recurring infection by RS viruses every year. Voluntary studies with adults have demonstrated
that a reinfection with RS viruses is possible. The incubation time is 2 - 6 days. RS viruses are
paramyxoviruses with a diameter of 90 - 140 nm. The most noticeable connection of RSV
infections with respiratory infections and specific clinical syndromes was detected in infants up to 6
months of age with bronchiolitis or pneumonia. In older infants or small children the disease is
milder. In 25 % of infections of the respiratory tract RSV infections are detectable. As reinfections
with RSV are possible, it is assumed that these reinfectious antibodies are responsible for the mild
course of the disease in adults, being similar to a cold. However, especially in the early years,
serum antibodies are no effective protection against infections of the respiratory tract. Therefore,
this pathogen may cause bronchiolitis or, in infants up to 4 months of age, pneumonias. Based on
their antigen relationship, RSV isolates are differenciated into two major groups (A and B). The
surface glycoproteins of the virus (G glycoprotein and fusion glycoprotein) cause the production of
virus-neutralizing antibodies. Obviously the G glycoproteins of groups A and B are very different,
while the F glycoproteins of both groups show a high antigen concurrence. The complement
binding reaction is unsatisfactory for the serological diagnosis of RSV. Enzyme immunoassays are
of diagnostic value for the serological diagnosis of RSV infections, as they are very sensitive and
allow the differentiation of antigens into the various immunoglobulin classes. In RSV infections, it
is possible that the IgM antibody response is missing or so weak that a reliable interpretation of the
results is impossible. The detection of IgG antibodies in a single sample is no evidence for an acute
infection as, in some patient IgA, antibodies may persist months and years. The method
recommended for serological testing of acute RSV infections is the determination of IgG antibodies
in serum pairs with significant titer rise.

The Diagnostic Automation RSV IgG antibody test kit is based on the principle of the enzyme
immunoassay (EIA). RSV antigen is bound on the surface of the microtiter strips. Diluted patient
serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding
between the IgG antibodies of the serum and the immobilized RSV antigen takes place. After a one
hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to
remove unbound material. Then ready-to-use anti-human-IgG peroxidase conjugate is added and
incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and
incubated for 20 minutes, inducing the development of a blue dye in the wells. The color
development is terminated by the addition of a stop solution, which changes the color from blue to
yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The
concentration of the IgG antibodies is directly proportional to the intensity of the color.

Product Note:

Our RSV IgG Antibody ELISA Test Kit has been designed for the the detection and the quantitative determination of specific IgG antibodies against Respiratory syncytial virus in serum and plasma.