Mycobacterium Tuberculosis IgA (TB) ELISA kit description:

The Diagnostic Automation Mycobacterium tuberculosis IgA antibody ELISA kit has been
designed for the detection and the quantitative determination of specific IgA antibodies against
Mycobacterium tuberculosis in serum and plasma. Further applications in other body fluids are
possible and can be requested from the Technical Service of Diagnostic Automation.This assay is
intended for in-vitro diagnostic use only. Laboratory results can never be the only base of a medical
report. The patient history and further tests have additionally to be taken into account.

Mycobacterioses (tuberculosis, leprosy, atypical mycobacterioses, paratuberculosis, and perhaps
Crohn’s Disease) are the infectious diseases of men and animals with the largest diffusion on earth.
The infectious agents of tuberculosis are acid-resistant rod-like formed bacteria of the family
Mycobacteriaceae, genus Mycobacterium. The germ was detected by Robert Koch in 1882. Owing
to the very high infectious power of pathogenic mycobacteria, early diagnosis is essential to prevent spreading of the disease. Convergence of various approaches are necessary to control the
mycobacterioses, immune reactions and bacterial shedding being variable during the diseases.
However, usual diagnostic procedures were up to now unsatisfactory and did not allow to distinguish among different mycobacterial species. The illness is normally transferred by droplets of saliva from infected persons. This ELISA test kit for the determination of IgG antibodies uses a cocktail of highly pure proteins in order to determine an immune response against the bacteria in human serum. A fresh or chronically active infection can be diagnosed by IgA and IgM tests, which are also available.

The Diagnostic Automation Mycobacterium tuberculosis IgA antibody test kit is based on the
principle of the enzyme immunoassay (EIA). Mycobacterium tuberculosis antigen is bound on the
surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the
wells of the microtiter plate. A binding between the IgA antibodies of the serum and the immobilized Mycobacterium tuberculosis antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgA peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of IgA antibodies is directly proportional to the intensity of the color.