Lyme Disease IgM ELISA Test description:

Lyme Disease IgM ELISA Test Kit is an enzyme-linked immunosorbent test for the qualitative detection of IgM antibodies to Borrelia burgdorferi in human serum.




Materials Provided with B burgdorferi IgM elisa kit:
1. Plate: wells coated with inactivated B burgdorferi (B31 strain) antigen
2. HRP-Conjugate goat anti-human IgM
3. Positive Control: Human Serum
4. Calibrator: Human Serum
5. Negative Control: Human Serum
6. Absorbent Solution: goat anti-human
7. Sample Diluent
8. TMB
9. Stop Solution
10. Wash Buffer Concentrate 10X
11. Package Insert

Materials & Instrumentations Required, not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette & tips
3. Dry incubator or water bath
4. Microshaker for dissolving and mixing conjugate with samples
5. EIA kit Microplate Washer
6. EIA kit Microplate Reader at 450nm wavelength




Lyme Disease elisa kit Background Information:
Borrelia burgdorferi is a spirochete that causes Lyme disease. The organism is transmitted by ticks of the genus Ixodes. Patients with early infection produce IgM antibodies during the first few weeks after onset of erythema migrans (EM) and produce IgG antibodies more slowly. Although IgM only may be detected during the first month after onset of illness, the majority of patients develop IgG antibodies within one month. Both IgG and IgM antibodies can remain detectable for years. Serological testing methods for antibodies to B. burgdorferi include indirect fluorescent antibody (IFA) staining, immunoblotting, and enzyme immunoassay (EIA). B. burgdorferi is antigenically complex with strains that vary considerably. Early antibody responses often are to flagellum which has cross reactive components. Patients in early stages of infection may not produce detectable levels of antibody. Also, early antibiotic therapy after EM may diminish or abrogate good antibody response. Some patients may never generate detectable antibody levels. Thus, serological tests for antibodies to B. burgdorferi are known to have low sensitivity and specificity and because of such inaccuracy, these tests for Lyme disease cannot be relied upon for establishing a positive diagnosis.

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Lyme Disease ELISA Test Principle:
Borrelia burgdorferi ELISA test kit is designed to detect IgM class antibodies to B. burgdorferi in human sera. Wells of plastic microwell strips are sensitized by passive absorption with B. burgdorferi antigen. The test procedure involves three incubation steps. For additional details please refer to the instructions for use.





This Lyme Disease elisa kit is designed to detect IgM class antibodies to B. burgdorferi in human sera. Diagnostic Automation provides two other Lyme Disease ELISA Test Kits:
1. Lyme Disease IgG ELISA Test Kit, designed to detect IgG antibodies to B. burgdorferi in human sera.
2. Combination Lyme Disease IgG/IgM ELISA Test Kit, designed to detect IgM and IgG class antibodies to B. burgdorferi in human sera.

For more information about these Lyme Disease ELISA Test Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .