Lyme Disease IgM ELISA kit
Lyme Disease IgM ELISA kit
|Category Name||Infectious Disease ELISA kits|
|Method||ELISA method: Enzyme Linked Immunosorbent Assay|
|Principle||Indirect ELISA: Antigen Coated Plate|
|Detection Range||Qualitative Positive; Negative control & Cut off|
|Total Time||~70 min|
|Shelf Life||12-18 Months from the manufacturing date|
Los Angeles-based Diagnostic Automation/Cortez Diagnostics, Inc. offers a Lyme Disease ELISA Test, known as Lyme Disease IgM ELISA Test Kit, which is an enzyme-linked immunosorbent ELISA test for the qualitative presumptive detection of IgM antibodies to Borrelia burgdorferi in human serum. This Lyme Disease ELISA Test should only be used for patients with signs and symptoms that are consistent with Lyme disease. The Lyme Disease ELISA Test is for in-vitro diagnostic use only.
The Lyme Disease ELISA Test system is designed to detect IgM class antibodies to B. burgdorferi in human sera. Wells of plastic micro well strips on this Lyme Disease Test are sensitized by passive absorption with B. burgdorferi antigen. The Lyme Disease test procedure involves three incubation steps: (1) Test sera are diluted with the sample diluent provided. The sample diluent on this contains anti-human IgG which precipitates and removes IgG and rheumatoid factor from the sample leaving IgM free to react with the immobilized antigen on the Lyme Disease Test. During sample incubation, any antigen specific IgM antibody in the sample will bind to the immobilized antigen. The plate is washed to remove unbound antibody and other serum components. (2) Peroxidase conjugated goat anti-human IgM is added to the wells of the test and the plate is incubated. The conjugate will react with IgM antibody immobilized on the solid phase in step 1. The wells of the are washed to remove unbound conjugate. (3) The microwells of the Lyme Disease Test containing immobilized peroxidase conjugate, are incubated with peroxidase substrate solution. Hydrolysis of the substrate by peroxidase produces a color change. After a period of time, the reaction is stopped and the color intensity of the solution is measured photometrically. The color intensity of the solution on the Lyme Disease Test depends upon the antibody concentration in the original test sample.
It is recommended that specimen collection be carried out carefully. No known test method can offer complete assurance that human blood samples will not transmit infection. Therefore, all blood derivatives for this Lyme Disease ELISA Test should be considered potentially infectious. Only freshly drawn and properly refrigerated sera obtained by approved aseptic venipuncture procedures should be used in this Lyme Disease Test. No anticoagulants or preservatives should be added. Avoid using hemolyzed, lipemic, or bacterially contaminated sera on the Lyme Disease Test. Store sample at room temperature for no longer than 8 hours. If testing with this Lyme Disease Test is not performed within 8 hours, sera maybe e stored between 2 and 8 degrees Celsius, for no longer than 48 hours. If delay in testing is anticipated, store the sera at -20 degrees Celsius or lower. Avoid multiple freeze/thaw cycles that may cause loss of antibody activity and giver erroneous results on this Lyme Disease Test.
A negative is indicated by no detectable IgM antibody. The result does not exclude B. burgdorferi infection. An additional sample for this Lyme Disease ELISA Test should be tested within 4-6 weeks if early infection is suspected. A positive indicates IgM antibody to B. burgdorferi is detected. See Lyme Disease ELISA Test kit insert for more information about following specific procedures, establishing quality control, and calculating test results.
Diagnostic Automation/Cortez Diagnostics, Inc. also provides two other Lyme Disease ELISA Tests. One Lyme Disease Test is the Lyme Disease IgG ELISA Test Kit which is an ELISA test system designed to detect IgG antibodies to B. burgdorferi in human sera. The other test is the combination Lyme Disease IgG/IgM ELISA Test Kit which is designed to detect IgM and IgG class antibodies to B. burgdorferi in human sera. For more information about these Lyme Disease ELISA Test Kits, please contact our Customer Service Department at 818-591-3030.