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Lyme Disease ELISA Test

Name

Lyme Disease IgM ELISA kit

Full name

Human Lyme Disease IgM ELISA Test kit

Category Name Infectious Disease ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle Indirect ELISA: Antigen Coated Plate
Detection Range Qualitative Positive; Negative control & Cut off
Sample 10 ul serum
Specificity 92.50%
Sensitivity 95.50%
Total Time ~70 min
Shelf Life 12-18 Months from the manufacturing date

Item #:                    1424-2   Quantity:               

Lyme Disease ELISA Test

Lyme Disease IgM_1424-2Z _MSDS_ 2-4-2010.pdf  

Lyme Disease ELISA Test

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Lyme Disease ELISA Test description:




Diagnostic Automation Inc. offers a Lyme Disease ELISA Test, known as Lyme Disease IgM ELISA Test Kit which is an enzyme-linked immunosorbent ELISA test for the qualitative presumptive detection of IgM antibodies to Borrelia burgdorferi in human serum. This Lyme Disease ELISA Test should only be used for patients with signs and symptoms that are consistent with Lyme disease. The Lyme Disease ELISA Test is for in-vitro diagnostic use only.




Materials provided with B burgdorferi IgM elisa kit:

1. Plate: 96 wells, strips coated with inactivated B burgdorferi (B31 strain) antigen
2. HRP-Conjugate goat anti-human IgM
3. Positive Control: Human Serum
4. Calibrator: Human Serum
5. Negative Control: Human Serum
6. Absorbent Solution: goat anti-human
7. Sample Diluent: containing Tween-20, bovine serum albumin
8. TMB: containing 3, 3’, 5, 5’ –tetramethylbenzidine (TMB)
9. Stop Solution: containing 1M H2SO4, 0.7MHCI
10. Wash Buffer Concentrate (10X): containing a 10X
concentrated PBS & Tween-20 solution
11. Package Insert, 1 copy


Materials & Instrumentations Required, not provided:

1. Freshly distilled or deionized water
2. Dispensing system and/or pipette & tips
3. Dry incubator or water bath, 37± 0.5°C
4. Microshaker for dissolving and mixing conjugate with samples
5. EIA kit Microplate Washer
6. EIA kit Microplate Reader at 450nm wavelength 


B burgdorferi IgM elisa kit Background Information
Borrelia burgdorferi is a spirochete that causes Lyme disease. The organism is transmitted by ticks of the genus Ixodes. In endemic areas, these ticks are commonly found on vegetation and animals such as deer, mice, dogs, horses, and birds. B. burgdorferi infection shares features with other spirochetal infections (diseases caused by three genera in humans: Treponema, Borrelia, and Leptospira). Skin is the portal of entry for B. burgdorferi and the tick bite often causes a characteristic rash called erythema migrans (EM). EM develops around the tick bite in 60% to 80% of patients. Spirochetemia occurs early with wide spread dissemination through tissue and body fluids. Lyme disease occurs in stages, often with intervening latent periods and with different clinical manifestations. In Lyme disease there are generally three stages of disease often with overlapping symptoms. Symptoms vary according to the sites affected by the infection such as joints, skin, central nervous system, heart, eye, bone, spleen, and kidney. Late disease is most often associated with arthritis or CNS syndromes. Asymptomatic subclinical infection is possible and infection may not become clinically evident until the later stages. Patients with early infection produce IgM antibodies during the first few weeks after onset of EM and produce IgG antibodies more slowly. Although IgM only may be detected during the first month after onset of illness, the majority of patients develop
IgG antibodies within one month. Both IgG and IgM antibodies can remain detectable
for years.
Serological testing methods for antibodies to B. burgdorferi include indirect fluorescent antibody (IFA) staining, immunoblotting, and enzyme immunoassay (EIA). B. burgdorferi is antigenically complex with strains that vary considerably. Early antibody responses often are to flagellum which has cross reactive components. Patients in early stages of infection may not produce detectable levels of antibody. Also, early antibiotic therapy after EM may diminish or abrogate good antibody response. Some patients may never generate detectable antibody levels. Thus, serological tests for antibodies to B. burgdorferi are known to have low sensitivity and specificity and because of such inaccuracy, these tests cannot be relied upon for establishing a diagnosis of Lyme disease. In 1994, the Second National Conference on Serological diagnosis of Lyme disease recommended a two-step testing system toward standardizing laboratory serologic testing for B. burgdorferi.


 
This Lyme Disease elisa kit is designed to detect IgM class antibodies to B. burgdorferi in human sera. Diagnostic Automation Inc. provides two other Lyme Disease ELISA Test Kits:
1. Lyme Disease Test is the Lyme Disease IgG ELISA Test Kit which is an ELISA test system designed to detect IgG antibodies to B. burgdorferi in human sera. 
2. Combination Lyme Disease IgG/IgM ELISA Test Kit which is designed to detect IgM and IgG class antibodies to B. burgdorferi in human sera. 
For more information about these Lyme Disease ELISA Test Kits, please contact our Customer Service Department at 818-591-3030.



Product Note:

Borrelia burgdorferi (Lyme Disease IgM) ELISA test kit system is designed to detect IgM class antibodies to B. burgdorferi in human sera. Wells of plastic microwell strips are sensitized by passive absorption with B. burgdorferi antigen.