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PSA ELISA kit

Name

PSA ELISA kit

Full name

Human PSA ELISA Test Kit

Category Name Cancer ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA priciple- Peroxidase conjugated
Detection Range 0-100 ng/mL
Sample 50 ul serum
Specificity 98.7%
Sensitivity 0.5ng/mL
Total Time ~140 min
Shelf Life 12-14 Months from the manufacturing date

Item #:                    4222-16   Quantity:               

PSA ELISA kit


PSA ELISA kit

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PSA ELISA kit description:




PSA ELISA test kit is an Enzyme Immunoassay which provides a quantitative measurement of PSA in human serum.




Material Provided with PSA ELISA Kit:
1. Microtiter wells coated with PSA ELISA Test Antibody
2. PSA EIA Test Kit Zero buffer
3. PSA EIA Reference standard containing 0, 2, 4, 15, 50 & 100 ng/ml
4. PSA ELISA Test Enzyme Conjugate Reagent
5. PSA Elisa TMB Substrate
6. Stop Solution
7. PSA Test Kit Wash Buffer Concentrate 50X

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm




PSA ELISA Kit Background Information:
Human prostate-specific antigen (PSA) is a serine protease, a single chain Glycoprotein. PSA is immunologically specific for prostatic tissue, it is present in normal, benign hyperplastic, and malllignant prostatic tissue, in metastatic prostatic carcinoma, and also in prostatic fluid and seminal plasma. PSA is not present in any other normal tissue obtained from men, nor is it produced by cancers of the breast, lung, colon, rectum, stomach, pancreas or thyroid. PSA is functionally and immunologically different from prostatic acid phosphatase (PAP). Serum PSA is one of the most useful tumor markers in oncology. It may serves as an accurate marker for assessing response to treatment in patients with prostatic cancer. Therefore, measurement of serum PSA concentrations can be an important tool in monitoring patients with prostatic cancer and in determining the potential and actual effectiveness of surgery or other therapies. Recent studies also indicate that PSA measurements can enhance early prostate cancer detection when combined with digital rectal examination.




PSA ELISA Test Principle:
The PSA ELISA test is a solid phase two-site immunoassay. Rabbit anti-PSA is coated on the surface of the microtiter wells and another anti-PSA monoclonal antibody labeled with HRP is used as the tracer. The PSA molecules present in the standard solution or serum are sandwiched between the two antibodies. After incubation and washing, the enzymatic reaction develops a color which is proportional to the amount of PSA molecules present in the assay. For additional details please refer to the instructions for use.




Notable features of Cancer ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for all Cancer ELISA kits follow a similar method. See the PSA ELISA Kit product insert for specific details on preparation, procedures, quality control, and test result interpretation.


Diagnostic Automation Inc. also provides other Autoimmune Disease ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .





Product Note:

In this PSA elisa test kit the wash procedure is critical. Insufficient washing will result in a poor precision and falsely elevated absorbance readings.