Dengue IgG ELISA kit description:

 

Los Angeles based Diagnostic Automation/Cortez Diagnostics, Inc. offers a Dengue IgG ELISA kit which is a semi-quantitative enzyme immunoassay for the detection of antibodies to dengue in samples of human serum.   This Dengue IgG ELISA Kit is intended to be run by trained medical technologists only, in a hospital or clinical lab setting.

 

The Dengue IgG ELISA test kit is a highly sensitive and reliable assay to detect dengue antibodies. The microwells of the Dengue IgG ELISA kit are coated with purified dengue virus antigen from Vero cell cultured type 1-4 dengue.  During the first incubation with the diluted patients’ sera, any antibodies on the Dengue IgG ELISA test kit which are reactive with the antigen will bind to the coated wells. After washing to remove the rest of the sample on the Dengue IgG ELISA test, the Enzyme Conjugate is added. If antibodies have been bound to the wells of the Dengue IgG ELISA test, the Enzyme Conjugate will then bind to these antibodies. After another series of washes, a chromogen -tetramethylbenzidine or TMB- is added to the Dengue IgG ELISA test kit.  If the Enzyme Conjugate is present on the Dengue IgG ELISA test, the peroxidase will catalyze a reaction that consumes the peroxide and turns the chromogen from clear to blue. Addition of the Stop Solution on the Dengue IgG ELISA test ends the reaction and turns the blue color to a bright yellow color. The reaction on this Dengue IgG ELISA test may then be read visually or with an ELISA reader.

 

Dengue fever, caused by any of the four serotypes of dengue virus is endemic in Southeast Asia and South and Central America. Repeat infection with a second type of dengue virus is thought to cause dengue hemorrhagic fever in about 10 percent of infected people. Dengue antibodies do not confer immunity beyond 3-6 weeks to a second dengue type.  Infection with dengue virus can result in a wide disease spectrum, from a mild fever to life-threatening dengue hemorrhagic fever and dengue shock syndrome. Symptoms of classical dengue fever, following a 5-8 day incubation period, include rash, severe headache, nausea, vomiting, chills, malaise, macular rash and may include lymphadenopathy. Hemorrhagic dengue fever involves increased blood vessel permeability which can lead to shock and death in about 10% of reported cases. Dengue fever can only be treated by supportive care and is prevented by mosquito control.

 

In primary infections, circulating IgM antibody to the viral coat proteins is detected 5-6 days after the onset of illness, and gradually decreases within 1-2 months of onset. IgG antibody to dengue virus is detected approximately 14 days after onset in primary infections, and by day 2 in secondary infections. In secondary infections, IgM antibody may reappear but gradually diminishes, while IgG antibody persists, often at high titer. These patterns of dengue antibody development permit serological differentiation of primary and secondary infections. Characteristically, acute patients with primary infections have a higher IgM:IgG ratio than are found in secondary infections. Patients with secondary infections generally have higher IgG levels. Acute or recent infections are identified by a rise in antibody titer as well as high IgM levels.

 

The Diagnostic Automation/Cortez Diagnostics, Inc.'s Dengue IgG ELISA Kit provides an excellent methodology for specifically detecting antibodies to dengue virus in human serum.  The Dengue ELISA Kits are available in several formats:  Dengue IgG ELISA Kit, Dengue IgM ELISA Kit, Dengue IgG/IgM ELISA Kit, and Dengue NS1 ELISA Kit for detection of NS1 antigens in human serum.  For more information on any of the Dengue ELISA Kits or Dengue NS1 ELISA Kit, please contact our Customer Service Department.