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Echinococcus IgG ELISA kit

Name

Echinococcus IgG ELISA Test

Full name

Human Echinococcus IgG ELISA Test Kit

Category Name Parasitology ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle Sandwich Complex
Detection Range Qualitative elisa assay- Positive, Negative Controls
Sample 5 ul serum
Specificity 100%
Sensitivity 100%
Total Time ~20 min
Shelf Life 12 Months from the manufacturing date

Item #:                    8202-35   Quantity:               

Echinococcus IgG ELISA kit

Echinococcus_8202-35(MSDS)(05-18-2016).pdf

Echinococcus IgG  ELISA kit

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Echinococcus IgG ELISA kit description:




Echinococcus ELISA Test is an enzyme linked immunosorbent assay (ELISA) for the qualitative identification of IgG antibodies to Echinococcus in serum.




Material Provided with Echinococcus ELISA kit:
1. Test Strips Microwells: 96 test wells containing Echinococcus antigens
2. Enzyme Conjugate: Protein A conjugated to peroxidase
3. Positive Control: diluted diluted positive rabbit serum
4. Negative Control: diluted negative human serum
5. TMB Substrate Solution: TMB
6. Wash Concentrate 20X
7. Dilution Buffer
8. Stop Solution: 0.73 M phosphoric acid

Materials Required, Not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm and a 620 - 650 nm filter




Echinococcus ELISA kit Background Information:
Echinococcosis (hydatidosis) is the infection caused by cestodes of the genus Echinococcus. Humans are potential intermediate hosts and can become infected by ingesting eggs passed in the feces of an infected animal. The resulting disease is called hydatidosis, or hydatid disease.
Four species are known pathogens of the disease: E. granulosus, E. multilocularis, E. oligarthrus and E. vogeli. The infection caused by E. granulosus is referred to as cystic hydatid disease (CHD) and results in cysts in various organs, especially the liver and lungs. These cysts may become quite large and contain hundreds or thousands of scoleces called hydatid sand. The degree of antibody response to these cysts will vary depending on their location and degree of calcification. Liver cysts typically produce a higher antibody response than lung cysts. Infection due to E. multilocularis is referred to as alveolar hydatid disease (AHD), and also occurs as cysts that may spread throughout the infected tissue.




Echinococcus ELISA Test Principle:
The principle of the Echinococcus ELISA test involves three incubation steps. Patients sera are added to the microwells coated with Echinococcus cyst antigen, if any antibodies present, they
will bind to the wells during the first incubation. After wash Enzyme Conjugate is added which bind to any antibodies present. Then a chromogen is added and color change. The blue color turns to a bright yellow color after the addition of the stop solution, which ends the reaction.




Notable features of the Parasitology ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Parasitology ELISA kits follow a similar method. See the Echinococcus ELISA Test Kit product insert for details on preparation, procedures, quality control, and test result interpretation.



Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about these ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.


Product Note:

Since Echinococcus eggs are not shed by infected humans, serological determination

has been important in the diagnosis of hydatid disease. A number of tests have been

used, including latex agglutination (LA), indirect hemagglutination (IHA), complement

fixation (CF), agar gel diffusion (AGD) and enzyme linked immunosorbent assay (ELISA).