Japanese Encephalitis IgG ELISA kit description:

The Diagnostic Automation Inc. Japanese Encephalitis IgG ELISA test kit is for the exposure to Japanese Encephalitis Virus (JEV) is a assay system for the detection of antibodies in human serum to JEV derived recombinant antigen (JERA) (1-4). This test is to aid in the diagnosis of human exposure to the Japanese Encephalitis Virus (JEV). It is not intended to screen blood or blood components and is for professional in vitro diagnostic use only. This kit has not been optimized for vaccine induced
seroconversion studies.

SUMMARY AND EXPLANATION OF THE TEST

Exposure to JEV causes a disease with a number of symptoms including encephalitis (5-8). The
Japanese Encephalitis IgG ELISA IgG assay employs a recombinant antigen called JERA, which can
be used as a rapid serological marker for JEV infection. The JERA protein is a recombinant antigen,
which consists of a stretch of peptides from different parts of the JEV antigens.

PRINCIPLE OF THE TEST

The Diagnostic Automation Inc. Japanese Encephalitis IgG ELISA consists of one enzymatically amplified "two-step" sandwich-type immunoassay. In this assay, JE IgG Positive Control (Represents reactive or equivocally reactive serum), JE Negative Control (Represents non-reactive serum), and unknown serum samples are incubated in microtiter wells. The serum samples are diluted with Sample Dilution
Buffer for IgG. After incubation and washing, the wells are treated with an antibody specific for human IgG and labeled with the enzyme horseradish peroxidase (HRP). After a second incubation and
washing step, the wells are incubated with the Tetramethyl Benzidine (TMB) substrate.
An acidic stopping solution is then added and the degree of enzymatic turnover of the substrate is determined by absorbance measurement at 450 nanometers. Above a certain threshold, the ratio of the absorbencies of the JERA and the control wells accurately determines whether antibodies to JEV are present.