Rapid Plasma Reagin (RPR) set is a non-treponemal flocculation test (Serology Test kit) that is used for the qualitative and semi-quantitative determination of reagin antibodies in serum or plasma from persons with syphilis.
SUMMARY AND PRINCIPLE OF THE TEST
Treponema pallidum, the etiological agent of syphilis, produces at least two types of antibodies in human infections: treponemal antibodies that can be detected by Fluorescent Treponemal Antibody-Absorption (FTA-ABS) test1 and non-treponemal antibody (reagin) that can be detected by RPR antigen card test. 2
The antigen used in the kit is a modification of VDRL antigen, which contains micro-particulate charcoal to enhance the visual difference between a positive and negative result. If a specimen contains reagin, flocculation occurs with a coaglutination of carbon particles contained in the antigen suspension, which appears as black clumps. Non-reactive specimens appear as an even light gray homogenous suspension.