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Free Testosterone ELISA kit


Free Testosterone ELISA Test

Full name

Human Free Testosterone ELISA Test Kit

Category Name Steroid ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle Competitive Enzyme Immunoassay
Detection Range 0.06 - 100 pg/ml
Sample 20 ul serum
Specificity 100%
Sensitivity 0.06 pg/ml
Total Time ~ 75 min
Shelf Life 12-14 Months from the manufacturing date

Item #:                    2924-17   Quantity:               

Free Testosterone ELISA kit


Free Testosterone ELISA kit

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Free Testosterone ELISA kit description:

The Diagnostic Automation Free Testosterone ELISA Test kit is a Competitive immunoenzymatic colorimetric method for quantitative determination of Free Testosterone concentration in serum and plasma.

Material Provided with Free Testosterone ELISA Kit:
1. Free Testosterone EIA Kit Standards 6x
2. Conjugte: Testosterone-HRP conjugate
3. Free Testosterone EIA Test Control
4. Microplate: coated with anti-Testosterone IgG
6. Stop solution: Sulphuric acid 0.15 M
7. Wash Solution 10X
Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm

Free Testosterone ELISA Kit Background Information:
Testosterone is primarily secreted in the test of males and the ovaries of females although small amounts are secreted by the adrenal glands. In both males and females, it plays key roles in health and wellbeing. Testosterone in the blood is bound to SHBG (60 %) and in lower quantity to other protein such as albumin. Only the measurement of Free Testosterone (< 1% of Total Testosterone) permits the estimating of the hormone biologically active. Testosterone levels decline gradually with age in men. Free testosterone levels are elevated in women with hyperandrogenism associated with hirsutism in the presence or absence of polycystic ovarian disease. In addition, free testosterone measurements may be more useful than total testosterone in situations where SHBG is increased or decreased such as in hypothyroidism and obesity.

Free Testosterone ELISA Test Principle:
The Diagnostic Automation Free Testosterone EIA is an ELISA assay based on the principle of competitive binding Free Testosterone (antigen) in the sample competes with the antigenic Testosterone conjugated with HRP present in the Conjugate for binding to the antibodies anti-Testosterone coated on the microplate. After incubation the bound/free separation is performed by a simple solid-phase washing. After the enzyme substrate are added color development occurs, reaction is stopped and the absorbances are determined. Free Testosterone concentration in the sample is calculated through a calibration curve. For additional details please refer to the instructions for use.

Diagnostic Automation Inc. also provides other Steroid ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .

Product Note:

Diagnostic Automation Free Testosterone EIA kits are designed for the measurement of Free Testosterone in human serum and plasma. The Testosterone bound to proteins is not measured by this Free Testosterone ELISA kit since the bound Testosterone does not take part in this reaction, so it is washed away during the washing step. For total Testosterone measurement the ELISA our Testosterone kit is available.