Androstenedione (ASD) ELISA kit
|Category Name||Steroid ELISA kits|
|Method||ELISA method: Enzyme Linked Immunosorbent Assay|
|Principle||ELISA principle - Peroxidase Conjugated Competitive ELISA|
|Detection Range||0-10 ng/ml|
|Total Time||~80 min|
|Shelf Life||12-14 months|
This ELISA test kit is a competitive immunoenzymatic colorimetric method for quantitative determination of Androstenedione concentration in serum and plasma.
Androstenedione (also known as 4-androstenedione) is a 19-carbon steroid hormone produced in the adrenal glands and the gonads as an intermediate step in the biochemical pathway that produces the androgen testosterone and the estrogens estrone and estradiol. It is the common precursor of male and female sex hormones. Some androstenedione is also secreted into the plasma, and may be converted in peripheral tissues to testosterone and estrogens. Androstenedione has relatively weak androgenic activity, estimated at ~ 20% of testosterone. However, serum androstenedione levels often exceed testosterone in both normal and disease states. Secretion and production rates also exceed those of testosterone in women in whom significant extra-adrenal conversion of androstenedione to testosterone occurs. In premenopausal women the adrenal glands and ovaries each produces about half of the total androstendione (about 3 mg/day). After menopause, androstenedione production is about halved, primarily due to the reduction of steroid secreted by the ovary. Nevertheless, androstenedione is the principal steroid produced by the postmenopausal ovary. Measurement of serum androstenedione provides a useful marker of androgen biosynthesis. Elevated androstenedione levels have been demonstrated in virilizing congenital adrenal hyperplasia. Serum androstenedione levels are also increased in polycystic ovary syndrome, and in case of hirsutism in women. Elevated serum androstenedione levels may also occur in adrenal and ovarian virilizing tumors.
Androstenedione (antigen) in the sample competes with horseradish peroxidase Androstenedione (enzyme-labeled antigen), for binding onto the limited number of anti-Androstenedione coated on the microplates (solid phase). After incubation, the bound/free separation is performed by a simple solid-phase washing. The enzyme substrate (H2O2) and the TMB-substrate (TMB) are added. After an appropriate time has elapsed for maximum colour development, the enzyme reaction is stopped and the absorbance is determined. Androstenedione concentration in the sample is calculated based on a series by a set of standard. The colour intensity is inversely proportional to the Androstenedione concentration in the sample.