SHBG ELISA kit
|Category Name||Steroid ELISA kits|
|Method||ELISA method: Enzyme Linked Immunosorbent Assay|
|Principle||ELISA Principle - Peroxidase Conjugated Competitive ELISA|
|Sample||10 ul serum|
|Total Time||~ 60 min|
|Shelf Life||12-14 months|
Sex-hormone-binding globulin (SHBG) is a B-globulin that specifically binds steroid hormones. Its molecular weight is 86 kDa/mol. The major site of SHBG synthesis is thought to be the hepatocytes. Its production is regulated by androgen/estrogen balance, thyroid hormones, insulin and dietary factors, among others. SHBG is involved in the transport of sex steroids in plasma.
Its concentration is a major factor regulating their distribution between protein-bound and free states. Determination of SHBG concentration is mainly of importance in the evaluation of mild disorders of androgen metabolism and it allows identification of women with hirsutisms that are likely to respond to estrogen therapy. Testosterone/SHBGratios correlate well with both measured and calculated values for free testosterone, and help to discriminate between subjects with excessive androgen activity and normal individuals.
A monoclonal antibody specific to SHBG is immobilized on microwell plates, and another monoclonal antibody, also specific to SHBG, is conjugated with horseradish peroxidase (HRP). SHBG from the sample is bound to the plates. After a washing step, HRP conjugate is added. After a second washing step, enzyme substrate is added. The enzymatic reaction is proportional to the amount of SHBG in the sample. The reaction is terminated by adding stopping solution. Absorbance is measured on a plate reader.