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T-2 Toxin Elisa kit

Name

T-2 Toxin Elisa Test

Full name

Food ELISA Test Kit

Category Name Parasitology ELISA kits
Test 96
Principle Quantitative
Specificity N/A
Sensitivity 0.9-117 ppb
Total Time ~20 min
Shelf Life 12-14 Months from the manufacturing date

Item #:                    5149-8   Quantity:               

T-2 Toxin Elisa kit


T-2 Toxin Elisa kit

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T-2 Toxin Elisa kit description:




The Diagnostic Automation, Inc. T-2 Toxin ELISA represents a highly sensitive detection system and is particularly capable of the quantification of T-2 Toxin contaminations in cereals, beer, milk and meat.




Materials Provided with T-2 Toxin ELISA Kit:
1. Microtiter plate:12 strips with 8 breakable wells coated with antibody-binding protein
2. T-2 Toxin Standards (0, 0.5, 2.5, 10, 25, 50 ng/mL): 6 vials with 1 mL each
3. Conjugate (T-2 Toxin-Peroxidase): 6 mL
4. Anti-T-2 Toxin Antibody (rabbit): 6 mL
5. Substrate Solution (TMB): 15 mL
6. Stop Solution (0.5 M H2SO4): 15 mL
7. Sample diluent (PBS) 2x
8. Washing Solution (PBS + Tween 20): 60 mL as 10x
9. Plastic bag to store unused microtiter strips
10. Instruction Manual

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength




T-2 Toxin ELISA Test Kit Background Information:
T-2 Toxin in addition to deoxynivalenol, zearalenone, the fumonisines and other trichothecenes belongs to the fusarium toxins. These toxins are already produced on the field in consequence of a contact of the cereals by fusarium species. Acute toxic dosages can result in gastroenteritis, damage of bone marrow up to necroses of skin and respiratory passages.
T-2 toxin has a high stability against temperature and can therefore also be detected in bakery products. In Russia the legislator set limit values for T-2 Toxin in food between 50 and 100 ppb. The introduction of limit values in the European union is discussed since many years. Thus an observation of food and feed with respect to the concentration of T 2 Toxin is increasingly obligatory.



T-2 Toxin Test Principle:
The Diagnostic Automation, Inc. T-2 Toxin quantitative test is based on the principle of the enzyme-linked immunosorbent assay. An antibody binding protein is coated on the surface of a microtiter plate.




Diagnostic Automation Inc offers several popular Food Allergen ELISA Kits: Egg ELISA Kit, Soy Protein Residue Detection in Food ELISA Kit, Lupine ELISA Kit, Mustard ELISA Kit, Peanut ELISA Kit, Soy ELISA Kit, Almond ELISA Kit, Gluten ELISA Kit, Walnut ELISA Kit, and Milk Protein Residue Detection in Food ELISA Kit. For more information on any of these Food ELISA Test Kits please see our website home page, or contact our Customer Service Representative at 818-591-3030.



Product Note:

When assaying Diagnostic Automation, Inc.  T-2 Toxin ELISA test kit, full compliance of the following good laboratory practices will determine the reliability of the results:  Prior to beginning the assay procedure, bring all reagents to room temperature 20-25C.  All reagents should be mixed by gentle inversion or swirling prior to use. Do not induce foaming.  Once the assay has been started, all subsequent steps should be completed without interruption and within the recommended time limits.

Replace caps in all the reagents immediately after use. Do not interchange vial stoppers.  Use a separate disposable tip for each specimen to prevent cross-contamination.  All specimens and standards should be run at the same time, so that all conditions of testing are the same.  Do not mix components from different batches.  Do not use reagents after expiration date.  Check both precision and accuracy of the laboratory equipment used during the procedure including micropipette and ELISA microplate reader.