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Pregnancy Rapid tests

Rubella IgA ELISA Test

Name

Rubella IgA ELISA kit

Full name

Human Rubella IgA ELISA Test kit

Category Name TORCH Panel
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA principle- Indirect; Antigen Coated Plate
Detection Range Qualitative elisa assay- Positive, Negative and Cut-off
Sample 5ul
Specificity 99%
Sensitivity 100%
Total Time 90min
Shelf Life 12-18months

Item #:                    1300-11   Quantity:               

Rubella IgA ELISA Test


Rubella IgA ELISA Test

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Rubella IgA ELISA Test description:




Diagnostic Automation Rubella IgA Elisa Test Kit is intended for use in the detection of IgA antibody to the rubella virus. Rubella IgA is an accurate and sensitive method to detect Rubella antibody IgA isotype.




Material Provided with Rubella IgA ELISA Kit:
1.Rubella IgA EIA Kit Microwell Strips: purified Rubella antigen coated wells
2.Absorbent Solution
3.Washing Concentrate 10x
4.Rubella IgA Test TMB Chromogenic Substrate
5.Enzyme Conjugate Rubella IgA EIA
6.Cut-off Calibrator, Rubella A Index = 1
7.Rubella IgA Kit Negative Control
8.Rubella IgA Kit Positive Control
9.Rubella IgA EIA Stop Solution

Materials & Instrumentations required, not included:
1. Deionized water
2. EIA Kit Microplate Washer
3. EIA Kit Microplate Reader at 450




Rubella IgA ELISA Test Kit Background Information
Rubella also known as German measles is caused by Rubella virus which belongs to the Togavirus family. Togavirus is an enveloped virus with a single-stranded RNA genome. Generally rubella is considered a mild adolescence disease. However a maternal infection could be transmitted through the placenta to the fetus, causing congenital rubella or congenital rubella Syndrome in newborn. Congenital rubella may result in chronic cardiac disease, growth retardation, hepatosplenomegaly, auditory defects, blindness, cerebral malformations and other severe anomalies. The first trimester defect is assoiated with the highest risk of defects in organogenesis. Children born asymptomatic may develop these abnormalities later in life. To reduce risk of such severe complications, accurate serological methods must be performed to determine the serologic status of childbearing aged women. The Rubella antibody status of pregnant women are commonly determined at the first prenatal visit. Those without antibodies are monitored through early pregnancy for seroconversion. The antibodies present to Rubella may be of IgA, IgM, and IgG. The physiological function of IgA and its clinical implication is still unclear. Rubella IgA elisa test provides an accurate and sensitive method to detect Rubella IgA antibody.




Rubella IgA ELISA Test Principle
Purified rubella antigen is coated on the surface of microwells. Diluted patient serum is added to wells and the rubella IgA specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and TMB Chromogenic substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgA specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.




Rubella IgA Elisa Test Results Interpretation
Negative: Rubella A Index less than 0.90 are negative for IgA antibody to rubella virus
Positive: Rubella A Index of 1.00 or greater are positive for IgA antibody to rubella virus




Rubella IgA Elisa Test Performance Characteristics
The precision of the assay was evaluated by testing three different sera eight replicates on 3 days. The intra-assay and inter-assay C.V. are:
Intra-assay: Negative 7.6% Low positive 6.2% Positive 4.8%
Inter-assay: Negative 11.6% Low positive 8.6% Positive 6.8%




Rubella IgA ELISA Related Kits:

Rubella IgG ELISA Kit

http://www.rapidtest.com/index.php?i=Rubella-IgG-ELISA-kit&id=105&cat=16

Rubella IgM ELISA Kit

http://www.rapidtest.com/index.php?i=Rubella-IgM-ELISA-kit&id=106&cat=16


Rubella Chemiluminescence Immuno Assay (CLIA) Kits:

Rubella IgG CLIA kit

http://www.rapidtest.com/index.php?i=Rubella-IgG-CLIA-kits---(Chemiluminescence-Immuno-Assay)&id=550&cat=41


Rubella IgM CLIA kit

http://www.rapidtest.com/index.php?i=Rubella-IgM-CLIA-kits---(Chemiluminescence-Immuno-Assay)&id=551&cat=41 CLIA kits



Diagnostic Automation Inc. also provides other Test Kits:

http://www.rapidtest.com/index.php?product=Rapid-Tests


For more information about ELISA Kits, Rapid Tests, CLIA tests, or IFA Tests, please contact our Customer Service Representatives at 818-591-3030.


Product Note:

Our Rubella IgM Elisa kit have high specificity and sensitivity.