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Pregnancy Rapid tests

CMV IgG ELISA Kit

Name

CMV IgG ELISA Test

Full name

Human Cytomegalovirus IgG (CMV IgG) ELISA Test kit

Category Name TORCH Panel
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA principle- Indirect; Antigen Coated Plate
Detection Range Qualitative elisa assay- Positive, Negative and Cut-off
Sample 5uL Serum
Specificity 98%
Sensitivity 97%
Total Time ~75 min
Shelf Life 12 Months from the manufacturing date

Item #:                    1201-11   Quantity:               

CMV IgG ELISA Kit

CMV IgG_1201-11 (MSDS)(03-04-2014).pdf

CMV IgG ELISA Kit

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CMV IgG ELISA Kit description:




Diagnostic Automation Inc. offers a CMV IgG elisa test kit which is intended for use in the detection of IgG antibodies to Cytomegalovirus (CMV) infection.




Material Provided with Cytomegalovirus IgG ELISA Kit:
1.Microwell strips: purified CMV antigen coated wells
2. Cytomegalovirus IgG EIA Kit Enzyme conjugate
3. Negative Calibrator: 0 IU/ml
4.Cytomegalovirus IgG Test Cut-off Calibrator: 1.2 IU/ml
5.Positive Calibrator: 6 IU/ml
6.Positive Calibrator: 18 IU/ml
7.Cytomegalovirus IgG Elisa Test Negative Control
8.Positive Control
9.Washing concentrate 20x
10. Cytomegalovirus IgG EIA Kit Sample diluent
11. TMB Chromogenic Substrate
12. Stop solution

Materials & Instrumentations required, not included:
1. Reagent tubes for the serum dilution
2. Bidistilled water
3. Precision pipettes
4. ELISA Kit Microplate Washer
5. ELISA Kit Microplate Reader at 450





CMV IgG elisa test kit Background Information
Cytomegalovirus is a herpes virus with the ability to remain dormant in the body for a long period of time. However, severe impairment of immune system by medication or disease can reactivate the virus from the latent or dormant state. CMV is found universally throughout all geographic locations and socioeconomic groups. CMV is a leading biological factor causing congenital abnormalities and complications among those who receive massive blood transfusions and immunosuppressive therapy. About half of pregnant women who contract a primary infection spread the disease to their fetus. In-utero infection may cause major defects including mental retardation, blindness, and/or deafness. Serological tests for detecting the presence of antibody to CMV can provide valuable information regarding the history of previous infection, diagnosis of active or recent infection, as well as in screening blood for transfusions in newborns and immuno-compromised recipients. The antibodies present to Cytomegalovirus may be of the IgA, IgM and IgG. Diagnostic Automation CMV IgG elisa test kit is an accurate serologic method to detect CMV IgG antibody for identification of CMV infection.




CMV IgG elisa test kit Principle
Purified CMV antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the CMV IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and TMB Chromogenic Substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.




CMV IgG elisa test kit Results Interpretation
Negative: CMV G Index of 0.90 or less are seronegative for IgG antibody to CMV ( <1.1 IU/ml )
Equivocal: CMV G Index of 0.91-0.99 are equivocal. Sample should be retested
Positive: CMV G Index of 1.00 or greater, or IU value greater than 1.2 are seropositive. It
indicates prior exposure to the CMV virus. ( > 1.2 IU/ml )




CMV IgG elisa test kit Performance & Characteristics
Precision
The precision of the assay was evaluated by testing three different sera of eight replicates over 3 days. The intra-assay and inter-assay C.V. Summary are:
Intra-assay: Negative 8.9% Low positive 8.2% Positive 7.2%
Inter-assay: Negative 9.7% Low positive 8.3% Positive 7.8%




CMV IgG elisa test Related Kits:

CMV IgA elisa test kit
http://www.rapidtest.com/index.php?i=Cytomegalovirus-IgA-(CMV-IgA)-ELISA-Test&id=110&cat=16


CMV IgM elisa test kit

http://www.rapidtest.com/index.php?i=Cytomegalovirus-IgM-(CMV-IgM)-ELISA-kit&id=109&cat=16


Chemiluminescence Immuno Assay (CLIA) Kits:

CMV IgM CLIA Kit


http://www.rapidtest.com/index.php?i=CMV-IgM-CLIA-kits---(Chemiluminescence-Immuno-Assay)&id=545&cat=41

CMV IgG CLIA Kit

http://www.rapidtest.com/index.php?i=CMV-IgG-CLIA-kits---(Chemiluminescence-Immuno-Assay)&id=544&cat=41


CMV IFA (Immunofluorescence Assay) Kits:

CMV IgG IFA Kit

http://www.rapidtest.com/index.php?i=CMV-IgG-IFA-Kit---(Immunofluorescence-Assay)-&id=503&cat=36

CMV IgM IFA Kit

http://www.rapidtest.com/index.php?i=CMV-IgM-IFA-Kit---(Immunofluorescence-Assay)-&id=504&cat=36


HBsAg Rapid Test

http://www.rapidtest.com/index.php?i=HBsAg-Rapid-Test&id=352&cat=30



Diagnostic Automation Inc. also provides other Kits:

http://www.rapidtest.com/index.php?product=Rapid-Tests


For more information about ELISA Kits, Rapid Tests, CLIA tests, or IFA Tests, please contact our Customer Service Representatives at 818-591-3030.