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T3 ELISA kit


T3 ELISA test kit

Category Name Thyroid ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle Competitive ELISA
Detection Range 0-10ng/mL
Sample 50uL
Specificity 96.30%
Sensitivity 0.2 ng/mL
Total Time ~90min
Shelf Life 12 Months from the manufacturing date

Item #:                    3144-16   Quantity:               

T3 ELISA kit

T3 3144-16 (MSDS)(11-18-2013).pdf  

T3 ELISA kit

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T3 ELISA kit description:

For the quantitative measurement of total Triiodothyronine (T3) in human

Materials provided with T3 ELISA Test Kit:

1.Anti-T3 ELISA Antibody-Coated Microtiter Wells, 96 wells.
2.T3 HRPO Conjugate Concentrate, 0.8 ml.
3.T3 EIA Test Kit HRPO Conjugate Diluent, 15 ml
4.Reference Standard, 1 set. Ready to use.
5.T3 Test TMB Substrate, 12 ml
6.Stop Solution ,12 ml
7.T3 Elisa Test Kit Wash Buffer Concentrate (50X),15ml

Materials & Instrumentation required, not included:

1.Distilled water.
2.Precision pipettes: 0.05~ 0.2ml,1.0ml
3.Disposable pipette tips.
4.Vortex mixer or equivalent.
5.Absorbent paper.
6.Graph paper.
7.ELISA Kit Microplate Washer
8.ELISA Kit Microplate Reader at 450nm & 620nm wavelength

Background Information

The Human thyroid gland is a major component of the endocrine system. Thyroid hormones perform many important functions. They exert powerful and essential regulatory influences on growth, differentiation, cellular metabolism, and general hormonal balance of the body, as well as on the maintenance of metabolic activity and the development of the skeletal and organ system. The hormones thyroxine (T4) and 3, 5, 3’ thiiodothyronine (T3) circulate in the bloodstream, mostly bound to the plasma protein, thyroxine binding globulin (TBG). The concentration of T3 is much less than that of T-4, but its metabolic potency is much greater. T3 determination is an important factor in the diagnosis of thyroid disease. Its measurement has uncovered a variant of hyperthyroidism in thyrotoxic patients with elevated T3 values and normal T4 values. An increase in T3 without an increase in T4 is frequently a forerunner of recurrent thyrotoxicosis in previously treated patients. The clinical significance of T3 is also evident in patients in whom euthyroidism is attributable only to normal T3, although their T4 values are subnormal. T3 determination is also useful in monitoring both patients under treatment for hyperthyroidism and patients who have discontinued anti-thyroid drug therapy. It is especially valuable in distinguishing between euthyroid and hyperthyroid subjects. In addition to hyperthyroidism, T3 levels are elevated in women who are pregnant, and in women receiving oral contraceptives or estrogen treatment, paralleling TBG increases in a manner analogous to T4 levels. Likewise, a reduction in TBG concentration decreases T3 concentration. These changes in the T3 level, however, are not a true reflection of thyroid status.

In the T3 EIA, a certain amount of anti-T3 antibody is coated on microtiter wells. A measured amount of patient serum, and a constant amount of T3 conjugated with horseradish peroxidase are added to the microtiter wells. During incubation, the anti-T3 antibody is bound to the second antibody on the wells, and T3 and conjugated T3 compete for the limited binding sites on the anti-T3 antibody. After 60 minutes incubation at room temperature, the wells are washed 5 times by water to remove unbound T3 conjugate. A solution of TMB is then added and incubated for 20 minutes, resulting in the development of blue color. The color development is stopped with the addition of 2 N HCl, and the absorbance is measured spectrophotometrically at 450 nm. The intensity of the color formed is proportional to the amount of enzyme present and is inversely related to the amount of unlabeled T3 in the sample. By reference to a series of T3 standards assayed in the same way, the concentration of T3 in the unknown sample is quantified.
(T3) ELISA kit is FDA, CE and ISO certified.

Product Note:

Diagnostic Automation/Cortez Diagnostics T3 ELISA kit is based on the principle of a solid phase enzyme-linked immunosorbent assay (ELISA), designed and intended for the quantitative measurement of total Triiodothyronine (T3) in human serum.

Triiodothyonine hormone referred to T3 in Medical fields, is produced by the thyroid gland. Triiodothyonine has three iodine molecules attached to its molecular structure. Triiodothyonine is primarily responsible for regulation of metabolism, growth, digestive functions, brain development, muscle control, body temperature, and heart rate.

Since this hormone is essential in so many ways to human body, it is very important to be checked in patients. Both having too much or too little thyroxin can cause disorders such Hypothyroidism, when there is too little production of this hormone by the thyroid gland.

Symptoms can include tiredness, temperature sensitivity, low heart rate, weight gain, poor memory, depression, stiffness of the muscles and infertility.

Hyperthyroidism is another disorder when too much of this hormone is produced.

Symptoms include: weight loss, increased bowel movements, irregular menstrual cycle, rapid heartbeat, palpitations, irritability, tremor, and hair loss. Our T3 ELISA Test kit is fast, accurate and reliable test in detecting and quantifying T3 with sensitivity of 0.2 ng/ml. It has been approved by different regulatory bodies and is marketed and sold in many countries.

Please check our Thyroid ELISA kits category for more kits and information.

Other names used in industry for T3 ELISA kits are:

-Triiodothyroninne Elisa kit

-Human Triiodothyronine Elisa kit