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(T4) ELISA kit


(T4) ELISA kit

Category Name Thyroid ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle Competitive ELISA
Detection Range 0-30 ug/mL
Sample 50uL
Specificity 96.30%
Sensitivity 0.05 ug/mL
Total Time ~90 min
Shelf Life 12-14 Months from the manufacturing date

Item #:                    3149-16   Quantity:               

(T4) ELISA kit

T4 3149-16 MSDS(11-18-2013).pdf  

(T4) ELISA kit

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(T4) ELISA kit description:

Diagnostic Automation T4 ELISA kit is an enzyme immunoassay for the quantitative determination of Thyroxine (T4) in human serum.

Materials provided with T4 ELISA Test Kit:

1. T4 Test Reference standard set
2. Antibody-coated microtiter plate 96 wells
3. T4 EIA kit HRPO Conjugate Diluent, 15 ml
4. T4 EIA kit HRPO Conjugate Concentrate, 0.8 ml
5. TMB Substrate, 12 ml
6. T4 EIA Test Kit Stop Solution, 12 ml
7. T4 Test Wash Buffer Concentrate(50X),15ml

Materials & Instrumentations required, not included:

1. Precision pipettes & tips: 40ul~200ul and 1.0ml
2. Distilled water
3. Vortex mixer or equivalent
4. Absorbent paper or paper towel
5. ELISA Kit Microplate Washer
6. ELISA Kit Microplate Reader at 450nm wavelength

T4 EIA Kit Background Information
L-Thyroxine (T4) is a hormone that is synthesized and stored in the thyroid gland. Proteolytic cleavage of follicular thyroglobulin releases T4 into the bloodstream. Greater than 99% of T4 is reversibly bound to three plasma proteins in blood - thyroxine binding globulin (TBG) binds 70%, thyroxine binding pre-albumin (TBPA) binds 20%, and albumin binds 10%. Approximately 0.03% of T4 is in the free, unbound state in blood at any one time.
Diseases affecting thyroid function may present a wide array of confusing symptoms. Measurement of total T4 by immunoassay is the most reliable and convenient screening test available to determine the presence of thyroid disorders in patients. Increased levels of T4 have been found in hyper-thyroidism due to Grave’s disease and Plummer’s disease and in acute and subacute thyroiditis. Low levels of T4 have been associated with congenital hypothyroidism, myxedema, chronic thryoiditis (Hashimoto’s disease), and with some genetic abnormalities.

T4 EIA Test Principle
In the T4 EIA, a limited amount of anti-T4 antibody is coated on 96 wells microtiter plate. A measured amount of patient serum, and a constant amount of T4 conjugated with horseradish peroxidase are added to the microtiter wells. During incubation, T4 and conjugated T4 compete for the limited binding sites on the anti-T4 antibody. After a 60 minutes incubation at room temperature, the wells are washed 5 times by water to remove unbound T4 conjugate. A solution of TMB is then added and incubated for 20 minutes, resulting in the development of blue color. The color development is stopped with the addition of 2 N HCl, and the absorbance is measured spectrophotometrically at 450 nm. The intensity of the color formed is proportional to the amount of enzyme present and is inversely related to the amount of unlabeled T4 in the sample. By reference to a series of T4 standards assayed in the same way, the concentration of T4 in the unknown sample is quantified.

Expected values and sensitivity of T4 ELISA Kit
T4 EIA was performed in a study of 200 euthyroid patients in one geographic location and yielded a normal range of 5.0-13.0 ug/dl. This range corresponds to those suggested by other commercial manufacturers. It is recommended that laboratories adjust values to reflect geographic and population differences specific to their patients. The minimum detectable concentration of thyroxine by this assay is estimated to be 0.4ug/dl.

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Diagnostic Automation Inc. also provides other ELISA Kits. For more information about these EIA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.

Product Note:

Diagnostic automation/Cortez Diagnostics thyroxine (T4) ELISA test kit is intended for the quantitative determination of thyroxine (T4) in human serum. Measurement of total T4 by our T4 ELISA test is the most reliable and convenient screening test available to determine the presence of thyroid disorders in patients.

Our T4 ELISA test measures the blood level of the hormone T4, which is produced by the thyroid gland and helps control metabolism and growth. The T4 test maybe ordered by physicians for patients as part of an evaluation of thyroid function.  Our Total T4 measures the entire amount of thyroxine in the blood, including the amount attached to blood proteins that help transport the hormone through the bloodstream. Thyroid disorders include:

Hyperthyroidism which is an overactive thyroid typically associated with weight loss, a fast heart rate, and sweating.

Hypothyroidism which is an under-active thyroid, may cause symptoms such as weight gain, fatigue, and feeling cold. Newborns are routinely screened for hypothyroidism, which can cause mental disabilities if left untreated.

Our  high quality T4 ELISA kit is one of our best seller. It is FDA approved and CE marked. It has long history of sales in may countries around the world including USA.