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Total Human IgA ELISA kit

Name

Total Human IgA ELISA Test

Full name

Total Human IgA ELISA Test Kit

Category Name Allergy ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle Sandwich Complex
Detection Range 0.031 mg/mL - 2mg/mL
Sample 10ul serum or plasma
Specificity 94%
Sensitivity 0.031 mg/mL
Total Time ~150min
Shelf Life 12-14 Months from the manufacturing date

Item #:                    1802-9   Quantity:               

Total Human IgA ELISA kit


Total Human IgA ELISA kit

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Total Human IgA ELISA kit description:




The Total Human IgA ELISA Kit is for quantitative measurement of total human Immunoglobulin A (IgA) specific for common allergens in human serum and plasma.




Material Provided with Total Human IgA ELISA Kit:
1. Anti-Human IgA coated microwell strips
2. HRP conjugated goat anti-human IgA
3. Total Human IgA kit standard
4. TMB/peroxide substrate color developer
5. IgA ELISA kit specimen diluent
6. Sulfuric acid termination reagent 0.5N
7. 15 X Wash buffer concentrate

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm filter





Total Human IgA ELISA Kit Background Information:
Allergy is an immunological response often referred to as atopy. Most humans mount significant Immunoglobulin E responses only as a defense against parasitic infections. However, some individuals may respond to many common environmental antigens. This hereditary predisposition is called atopy. In atopic individuals, non-parasitic antigens stimulate inappropriate IgE production, leading to type I hypersensitivity. Sensitivities vary widely from one person to another. Hypersensitivity refers to an inappropriate or harmful immune-mediated response to an allergen. An allergen is a type of antigen that produces an abnormally vigorous immune response in which the immune system fights off a perceived threat that would otherwise be harmless to the body. A very broad range of substances can be allergens to sensitive individuals. Allergens fall into groups of proteins or proteases such as grasses, pollens, animal dander, house dust mites, and food to list a few. Exposure to allergens generally occurs at mucosal membranes in the respiratory and digestive tracts. The initial exposure to allergen is called sensitization and does not usually result in allergy symptoms. Subsequent secondary exposures to the same allergen allows for an immediate allergic response.
A variety of tests now exist to diagnose allergic conditions; these include skin and blood testing. Diagnostic Automation blood tests ELISA kits can be used for quantitative detection of IgE, IgM, IgA, or IgG specific mediated responses to known allergens. Diagnostic Automation ELISA based testing allows for the accurate analysis of hypersensitive-driven responses to known allergens of interest.




Total Human IgA ELISA Test Principle:
This Total Human IgA ELISA kit is IgA quantitative Test Kit based on a solid phase enzyme-linked immunosorbent assay. The assay system utilizes one anti-IgA antibody for solid phase (microtiter wells) immobilization and another anti-IgA antibody in the antibody-enzyme
horseradish peroxidase conjugate solution. For additional details please refer to the instructions for use.




Notable features of the Total Human ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for all Allergy ELISA kits follow a similar method. See the Total Human IgA ELISA Test Kit product insert for specific details on preparation, procedures, quality control, and test result interpretation.

Diagnostic Automation also provides other Human Allergen Specific ELISA kits: Human IgE ELISA Test kit, Human Allergen Specific IgM ELISA Test kit, and Human Allergen Specific IgG ELISA. Additionally, Total Human ELISA Kits are available: Total Human IgA ELISA Test kit, Total Human IgM ELISA Test kit, Total Human IgG ELISA Test kit, and Total Human IgA ELISA Test kit. For more information about ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .



Product Note:

The Total Human IgA ELISA Kit is based on Solid phase capture sandwich ELISA assay using a microwell format.