Toxoplasma IgG Chemiluminescence ELISA is intended for use in evaluating a patient's serologic
status to toxoplasma gondii infection.
Toxoplasmosis is caused by the intracellular parasite Toxoplasma gondii and may be contracted by
consuming contaminated meat or by contact with cat feces containing oocysts. In adolescence and
adulthood, most infections are subclinical. However, if a pregnant woman contracts toxoplasmosis, it
may be passed through the placenta to the fetus, resulting in congenital toxoplasmosis, which is a
cause of mortality and malformation. Asymptomatic infants may develop anomalies later in life.
Toxoplasma IgG Chemiluminescence ELISA is an accurate serologic method to detect Toxoplasma
antibody for clinical identification of toxoplasmosis.
Purified Toxoplasma gondii antigen is coated on the surface of microwells. Diluted patient serum is
added to wells, and the Toxoplasma gondii IgG specific antibody, if present, binds to the antigen. All
unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen
complex. Excess enzyme conjugate is washed off and substrate A & substrate B mixture is added. The light generated (RLU) is proportional to the amount of IgG specific antibody in the sample. The results are read by a microwell luminometer compared in a parallel manner with calibrator and controls.